An experimental work dealing with the gene modification using Cas9 RNA-based editing system was performed. Point site-specific breakpoints in gDNA were introduced at zygote stage by microinjection of spCas9 mRNA protein and guide RNAs into cytoplasm. Oocytes that extruded first second polar bodies used for injection. 2 series microinjections designs early bovine embryos made. The degeneration r...

The aim was to report a healthy live birth using re-vitrified-warmed cleavage-stage embryos derived from supernumerary warmed embryos after frozen embryo transfer (ET) in a patient with recurrent implantation failure (RIF). The case was a 39-year-old female with a history of polycystic ovarian syndrome and adenomyosis, along with RIF. After ovarian hyperstimulation, 33 cumulus-oocyte complexes ...

It is accepted that approximately 50% of embryos obtained after IVF arrest during the first week. Traditionally, chromosome abnormality and suboptimal culture conditions have been proposed as factors commonly associated with embryo arrest. However, even when considering 'ideal' conditions and embryos of only excellent morphology in vitro, there is still a significant incidence of embryonic arre...

STUDY QUESTION How accurate is array comparative genomic hybridization (array CGH) analysis of the first polar body (PB1) and second polar body (PB2) in predicting aneuploidies of maternal meiotic origin in the cleavage stage embryos of women of advanced maternal age? SUMMARY ANSWER Almost all of the aneuploidies detected in cleavage stage embryos were associated with copy number changes in t...