Agomelatine is a melatonergic anti-depressant. A stability-indicating high performance liquid chromatographic technique was developed for the determination of Agomelatine in pharmaceutical formulations. Chromatographic separation was achieved on Shimadzu Model CBM-20A/20 Alite, using Zorbax extended-C18 column (150 × 4.6 mm i.d., 5 μm particle size) with a mixture of 0.1% ammonium formate and a...

This enzyme immunoassay for specific measurement of secretory immunoglobulin A concentrations in human serum involves use of a small chromatographic column as a solid-phase. Serum samples are incubated for 2 h with beta-D-galactosidase-labeled antibody to secretory component, then passed through a 0.1-mL Sepharose 4B column containing antibodies to human immunoglobulin A. After the column is wa...

Ultra Performance Liquid Chromatography (UPLC) is a relatively new technique giving new possibilities in liquid chromatography, especially concerning decrease of time and solvent consumption. UPLC chromatographic system is designed in a special way to withstand high system back-pressures. The quality control analyses of various pharmaceutical formulations are transferred from HPLC to UPLC syste...

Uniform liquid-chromatographic conditions were developed such that we could quantify norepinephrine, epinephrine, normetanephrine, metanephrine, vanillylmandelic acid, and 5-hydroxyindoleacetic acid in urine by using a single mobile phase of monochloroacetic acid and citric acid, 0.1 mol/L each. All compounds were separated on a C18 column and detected electrochemically at a potential of +0.800...

An isocratic reversed-phase liquid chromatographic method was developed and validated for the determination of Solifenacin succinate. Chromatographic separation was achieved on a C18 column using an aqueous tetra butyl ammonium hydrogen sulphate (10 mM): acetonitrile (40:60, v/v), with flow rate 0.8 mL/min (UV detection at 254 nm). Linearity was observed in the concentration range of 20-200 μg/...

An efficient and simple one-pot approach for the synthesis of 3,4-dihydropyrimidin-2-(1H)-one derivatives using antimony trichloride (SbCl3) as a mild catalyst by means of three-component Biginelli reaction between β-keto esters, aldehyde derivatives and urea/thiourea under thermal and solvent-free conditions with excellent yields and short reaction times is reported. This m...

A simple and sensitive high performance liquid chromatographic method with electrochemical detection is described for the quantitative determination of the amoxycillin and clavulanic acid in pharmaceutical dosage forms. Separation of sample components occurs on a reversed phase C18 column with a mobile phase consisting of methanol-water-phosphate buffer. For the amperometric detection, the pote...

The column is the main component for chromatographic separation. Nowadays, monolithic columns are graining more popularity in field of separation media liquid chromatography. monolith possess great potential as compared to conventional packed terms preparing complex mixtures. These provide various properties like higher permeability, high-efficiency fast separations, high flow rate with lower b...

This article describe the applicability of 1,4-disulfopiperazine-1,4-diium chloride ([Piper-(SO3H)2].2Cl) as a green, versatile and Brönsted acidic ionic catalyst in the promotion of the synthesis of phthalazine derivatives via one-pot three component reaction between aromatic aldehydes, 1,3-diketone derivatives and phthalhydrazide under solvent-free reaction cond...

A simple and automated HPLC column-switching method with rapid sample pretreatment has been developed for quantitative determination of β-carotene in food supplements. Commercially samples of food supplements were dissolved in chloroform with help of saponification with 1M solution of sodium hydroxide in ultrasound bath. A 20-min sample dissolution/extraction step was necessary before chromatog...