Abstract The current study explores a design and development of the simple, fast, green selective novel method UPLC to quantify pitavastatin ezetimibe simultaneously. combined approach Green Analytical Method with Quality by Design-based risk assessment was done using Ishikawa fishbone diagram followed rotatable central composite used for optimization. optimal chromatographic separation attaine...

abstract: aqueous enzymatic extraction of oil from pistacia khinjuk was performed using cellulase. the central composite design was used to optimize the parameters that are significant to the process. the influence of three regressors on the percentage of oil recovery from seed was evaluated using second-order polynomial multiple regression model. analysis of variance showed a high coefficient ...

Many volatile compounds generated during the thermal processing of milk have been associated with cooked, stale, and sulfurous notes in milk and are considered as off-flavor by most consumers. A headspace solid-phase microextraction (HS-SPME)/gas chromatographic technique for the quantitative analysis of thermally derived off-flavor compounds was developed in this study. The extraction temperat...

The first experiment was a central composite rotatable design with calculated calcium levels of 6.2, 7.0, 9.0, 11.0, and 11.8 g/kg diet and total phosphorus levels of 5.2, 6.0, 8.0, 10.0, and 10.8 g/kg diet (2.8 g phytin-P/kg by analysis). This design involved three replicates for each rotatable point and fifteen replicates of the central point. The second experiment was a 4 x 4 factorial desig...

C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS) of polyethylene glycol (PEG)/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of PEG caused an increment in the purification factor for all the ATPS studied. Optimization of the...

Visceral leishmaniasis, a disease caused by Leishmania infantum chagasi, represents a major public health problem in many areas of the world. However, there is currently no vaccine for human use. The aim of this work was to purify the 503 antigen of Leishmania i. chagasi directly from unclarified Escherichia coli feedstock through expanded bed adsorption (EBA) chromatography. Batch experiments ...

Crude Maillard reactive product (MRP) and Maillard reactive product precursors (MRPP) of C. albidum pulp origin were extracted by varying temperature 0 C (30-70), time (min) (10-30 ) and addition of sodium carbonate solution (%) (2-10 mL) using the central composite rotatable designs. Equations for predicting objective indices namely; crude melanoidin, free amino acids, reducing sugar and relat...

Chickpea is considered among the most important leguminous crops in world but not a regular meal pulse due to their long cooking time and lengthy preparatory procedure. This gives rise need of converting raw chickpea into convenient food. For preparation instant from Desi Kabuli variety, Central Composite Rotatable Design (CCRD) under Response Surface Methodology (RSM) was employed which consis...

The dry gas seal (DGS) is a non-contacting, gas-lubricated mechanical face commonly used in rotating machinery. Traditional analyses of DGSs treat the end as an independent factor by setting end-face inlet boundary conditions, but limited attention focused on sealing chamber DGS. Using finite volume method and shear stress transport (SST) k-ω model, coupling between millimeter-scale micrometer-...