BACKGROUND Circulating cell-free DNA is present in increased amounts in the blood of patients with one of several forms of cancer. MATERIALS AND METHODS A real-time PCR assay with glutathione S-transferase pi (GSTP1) gene was used to measure cell-free DNA levels in the sera of 52 patients with hepatocellular carcinoma (HCC) associated with hepatitis C virus (HCV), which included 30 HCV carrie...

OBJECTIVE To investigate the prevalence of beta-cell autoantigen-reactive peripheral T-cells in type 1 diabetes, we developed an immunoglobulin-free enzyme-linked immunospot (ELISPOT) assay and assessed its usefulness for diagnosing this disease. RESEARCH DESIGN AND METHODS Cellular immune responses to beta -cell autoantigens were studied both by immunoglobulin-free proliferation assays and E...

Background and objectives: Luteolin (3′,4′,5,7-tetrahydroxy flavone) is one of the most common flavones, which is naturally found in several edible plants and traditional medicine. It is known as a non-toxic compound with anti-inflammatory, antinociceptive, anticarcinogenic, antimutagenic, and antiangiogenic properties. Luteolin has antiproliferative activity against different ...

introduction: auger electron based radioimmunotherapy (rit) using 111in-dota-trastuzumab (111in-dota-antiher2) feasibility was studied in vitro on a her2/neu positive cell line, skbr3. methods:111in-dota-antiher2 was prepared according to the optimized conditions followed by quality control tests including radiochemical purity; immunoreactivity). skbr3 as a her2/neu positive cell line was used ...

A recently derived in vitro chemiluminescence assay (Salles et al. [1995] Anal. Biochem., 232, 37-42) has been used to investigate the effects of a panel of twenty-two anticancer drugs and certain antibiotics on the excision repair activity of cell-free extracts from the human cell line, HeLa. This methodology, termed the 3D (Damaged DNA Detection) assay, based on the in vitro excision repair a...

aim and background: hepatocellular carcinoma (hcc) -an aggressive malignancy with high rate of mortality- is the third cause of cancer related death, worldwide. cisplatin is a chemotherapy drug used for treatment of hcc. however, serious side effects of drug limit the therapeutic administer dose.  nanotechnology based devices such as liposome nanoparticles are capable to overcome these drawback...

considering the emergence of antibiotic resistance, scientists are interested in using new antimicrobial agents in the treatment of infectious diseases including infections of the enteric systems. lactic acid bacteria have the great potential to produce antimicrobial compounds that inhibit and control pathogenic bacteria. the aim of this study was to determine the anti-bacterial and anti-adhere...

Background: The extracellular xylanase secreted by microorganisms is a hydrolytic enzyme, which arbitrarily cleaves the β-1, 4 backbone of the polysaccharide xylan; an enzyme used in the food processing, bio-pulping and bio-bleaching. The commercial production of the xylanase is limited because of a higher cost involvement, which can be overcome by the cost-effective production...

The biological application of nanoparticles (NPs) is a rapidly developing area of nanotechnology that raises new possibilities in the treatment of human cancers. The cytotoxicity was evaluated by MTT and LDH assays. The apoptotic effect of free ICD-85 and ICD-85 NPs on HeLa cells was assessed using caspase-8 colorimetric assay. The MTT assay showed that ICD-85 NPs could enhance the in-vitro cyt...