Carbapenem antimicrobials are critically important to human health and they are often the only remaining effective antibiotics for treating serious infections. Resistance to these drugs mediated by acquired carbapenemase enzymes is increasingly encountered in gram-negative bacteria and is considered a public health emergency. Animal origin food products are recognized as a potential source of r...

BACKGROUND AND OBJECTIVE The production of carbapenemases especially Klebsiella pneumoniae carbapenemase (KPC) is the most important mechanism of enzymatic resistance in isolated Enterobacteriaceae such as K. pneumoniae . The purpose of this study was detected of the carbapenemase producer K. pneumoniae strains with phenotypic and genotypic methods. METHOD Out of 800 strains, 270 K. pneumonia...

Carbapenemase-producing Enterobacteriaceae isolates are being increasingly reported, particularly from countries surrounding the Mediterranean area. We aimed to quantify the prevalence of carbapenemase- and extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in rectal swabs from hospitalized patients in a University hospital in Morocco, and to compare the performance of three scre...

The aim of this study was to identify the reason for frequent failure of carbapenems and β-lactam and β-lactamase inhibitor combination drugs in clinical settings despite susceptibility. For this different isolates collected from clinical specimens were tested for susceptibility and identification of carbapenemase producers was done by phenotypic method (Modified Hodge Test). Further, phenotypi...

et al. Quantitative nucleic acid sequence-based assay as a new molecular tool for the detection and quantifi cation of Leish-mania parasites in skin biopsies.ther observations on clinical, histopatho-logical, and immunological features of borderline disseminated cutaneous leish-maniasis caused by Leishmania (Leish-mania) amazonensis. leishmaniasis due to Leishmania (Leish-mania) amazonensis in ...

Evaluation of the Relative Frequency Carbapenemase Genes by Phenotypic and Genotypic Methods in Pseudomonas aeruginosa Isolates from Patients with Open Heart Surgery Iran

We report the first case of human infection due to Pseudomonas fulva. P. fulva caused acute meningitis following the placement of a drainage system in a 2-year-old female. Additionally, the isolate displayed a VIM-2 carbapenemase in a class 1 integron context.

A case of osteomyelitis in an infant following a burn injury sustained in Pakistan caused by a GES-13-producing Pseudomonas aeruginosa (the first reported in Canada) and an OXA-48 producing Klebsiella pneumoniae is described. The present case serves to highlight the importance of international travel as a risk factor for infection with carbapenemase-producing bacteria and the challenges in the ...

To rapidly identify carbapenemase producers in Enterobacteriaceae, we developed the Carba NP test. The test uses isolated bacterial colonies and is based on in vitro hydrolysis of a carbapenem, imipenem. It was 100% sensitive and specific compared with molecular-based techniques. This rapid (<2 hours), inexpensive technique may be implemented in any laboratory.