We report on a microfabricated device for sample preconcentration and capillary electrophoresis (CE) separation in a glass microchip. The device allows highly efficient anion-selective preconcentration to be performed with a micro-nanomicrochannel structure. An integrated cross channel enables gate-injection of the preconcentrated sample into the analysis channel for CE separation. Sample preco...

Copying multiple regions of a DNA molecule is routinely performed today using the polymerase chain reaction (PCR) in a process commonly referred to as multiplex PCR. The development of a multiplex PCR reaction involves designing primer sets and examining various combinations of those primer sets and different reaction components and/or thermal cycling conditions. The process of optimizing a mul...

The authors report a rare case of shock in a patient without significant clinical history, admitted to the intensive care unit for suspected septic shock. The patient was initially treated with fluid therapy without improvement. A hypothesis of systemic capillary leak syndrome was postulated following the confirmation of severe hypoalbuminemia, hypotension, and hemoconcentration--a combination ...

laxis are distinct clinical entities caused by transient plasma leakage from small blood vessels. When the affected vessels are superficial the result is a raised, itchy swelling of the skin known as a weal. Involvement of deeper vessels produces tender subcutaneous and submucosal swellings known as angio-oedema. Generalised plasma exudation leading to shock is a feature of anaphylaxis but also...

Systemic capillary leak syndrome (SCLS) is a rare disease characterized by third spacing of plasma into the extravascular compartment, leading to anasarca, hemoconcentration, and hypovolemic shock. It has been rarely associated with lymphomas, and reports usually indicate that it occurs after antineoplastic treatment. We present the case of a patient with ALK-negative anaplastic large cell lymp...

Treatment of acute renal allograft rejection with mouse monoclonal antibody (OKT3) is associated with systemic and neurologic side effects. We describe cerebral abnormalities in a 13-year-old boy with steroid-resistant renal allograft rejection. After treatment with OKT3, an acute neurologic syndrome developed, including seizures, lethargy, and decreased mental function. CT and MR imaging revea...

Cytotoxicity of drugs can be a cause of cardiorespiratory disorders connected with chemotherapy. Doxorubicin is an antibiotic from the group of anthracyclines effective in antineoplastic therapy of solid and hematopoetic tumors. The most common cause of therapy ceasing is its cardiotoxicity. However, a lung injury connected with its cytotoxic activity to pulmonary endothelium (capillary leak sy...

Capillary leak syndrome (CLS) commonly occurs in the intensive care setting. CLS is seen in conditions such as septic shock or may result from conditions such as multitrauma and pancreatitis, which result in the systemic inflammatory response syndrome (SIRS). We present two cases in which both patients suffered with CLS, which we believe was caused following administration of granulocyte colony...