We have developed a universal solid support, termed Rainbow Universal CPG, for use in automated oligonucleotide synthesis. The universal solid support allows any oligodeoxyribonucleotide sequence to be synthesized from a single type of controlled pore glass (CPG) support. Deprotection of oligodeoxyribonucleotides was optimized using 0.5 M LiCl in concentrated ammonium hydroxide. PCR experiments...

A comprehensive two-dimensional (2D) reverse phase (RP) liquid chromatography (LC) method is developed for alkaloid analysis. This offline comprehensive 2D method is developed using different pH values. With a pH value of 10.5, most alkaloids appear in the form of neutral molecules possessing high retention factors based on their polarity, while the alkaloid polarity order is changed when the p...

A high performance liquid chromatographic method for determination of Ethionamide (ETH) in human plasma was developed. The method involved deproteinisation of the sample with perchloric acid and analysis of the supernatant using a reversed-phase CN column (150mm) and a wavelength of 267 nm. The mobile phase consisted of Milli-Q water and Methanol (85:15, v/v) containing 0.05% Perchloric acid an...

Two simple, sensitive and economical spectrophotometric methods were developed for the determination of nifedipine in pharmaceutical formulations. Method A is based on the reaction of the nitro group of the drug with potassium hydroxide in dimethyl sulphoxide (DMSO) medium to form a coloured product, which absorbs maximally at 430 nm. Method B uses oxidation of the drug with ammonium molybdate ...

M2O3–TiO2 (M = Ga, In and La) composite oxides were prepared by a co-precipitation method with in situ generated ammonium hydroxide and were impregnated with 12 wt.% V2O5. The M2O3-TiO2 and V2O5/M2O3-TiO2 (M = Ga, In and La) samples were subjected to thermal treatments from 773 to 1073 K and were investigated by X-ray diffraction, FT-infrared, and BET surface area methods to establish the effec...

Due to its relatively low cost, high hydrogen yield, and environmentally friendly hydrolysis byproducts, magnesium hydride (MgH2) appears to be an attractive candidate for hydrogen generation. However, the hydrolysis reaction of MgH2 is rapidly inhibited by the formation of a magnesium hydroxide passivation layer. To improve the hydrolysis properties of MgH2-based hydrides we investigated three...

Monodisperse spherical silica particles were synthesized by mixing 40 ml of aqueous ammonia (NH4OH, 28 wt%) with a solution containing 1000 ml of absolute ethanol (EtOH) and 80 ml of deionized water (H2O). After stirring for 30 min, 60 ml of TEOS (98%, Aldrich) was added to the solution and stirred for 6 h at room temperature. The as-synthesized silica particle suspension was centrifuged and wa...

A method is reported for determining urinary xanthenuric acid (XA), a meta. bolite of tryptophan. XA is extracted from urine with isobutanol (2.methyl. 1.propanol), transferred to ammonium hydroxide, and separated from other urinary metabolites on thin.Iayer cellulose plates with use of 0.2 acetate buffer, mol/liter, pH 5.4. The XA is measured spectrophotometrically after diazotization with 2-c...