In the present study, we focused on the rostral ventrolateral medulla as a possible site of action for kinins because of its established importance in the central regulation of the cardiovascular system. Unilateral microinjections of 100 pmol to 4 nmol bradykinin into the rostral ventrolateral medulla produced dose-dependent increases in mean arterial pressure in Sprague-Dawley (SD) rats, Wista...

We studied the enhancement of the effects of bradykinin B2 receptor agonists by agents that react with active centers of angiotensin-converting enzyme (ACE) independent of enzymatic inactivation. The potentiation and the desensitization and resensitization of B2 receptor were assessed by measuring [3H]arachidonic acid release and [Ca2+]i mobilization in Chinese hamster ovary cells transfected t...

The cause of chest pain in angina pectoris and myocardial infarction is not yet clear at present. As one of its mechanisms Lewis, Katz, Raab and other authors ascribed it to the pain producing substances originated from the ischemic region of the myocardium. More recently bradykinin, which is known to have strong pain producing and hypotensive actions, was suggested by Burch and DePasqualel as ...

Bradykinin and (Thr(6))-bradykinin have been identified in the defensive skin secretion of the fire-bellied toad, Bombina orientalis. The homologous cDNAs for both peptides were cloned from a skin library using a 3'- and 5'-RACE strategy. Kininogen-1 (BOK-1) contained an open-reading frame of 167 amino acid residues encoding four repeats of bradykinin, and kininogen-2 (BOK-2) contained an open-...

Bradykinin concentrations in peripheral venous blood were measured in seven patients with carcinoid syndrome. The diagnosis was based on typical symptoms and raised urinary excretion of 5-hydroxy-3-indole acetic acid; the carcinoid tumour was verified histologically. Two patients were flushing constantly and the other patients had flushing attacks two to 10 times daily. Several blood samples we...

DOISIN are vasoactive polypeptides recently synthesized in pure form.1-3 Bradykinin and kallidin exist in man, whereas eledoisin has been found only in the posterior salivary glands of Eledone. Although the three poly-peptides have potent vasodilating and hypo-tensive properties, only bradykinin and kallid-in are closely related structurally (fig. 1). The nonapeptide structure of bradykinin was...

Bradykinin stimulates tissue plasminogen activator release from human endothelium through a flow-independent, B2 receptor-dependent mechanism. The present study tests the hypothesis that smoking impairs bradykinin-stimulated tissue plasminogen activator release. Graded doses of nitroprusside (1.6 to 6.4 microg/min), methacholine (3.2 to 12.8 microg/min), and bradykinin (100 to 400 ng/min) were ...

Intravital microscopic techniques were used to examine the mechanisms underlying bradykinin-induced leukocyte/endothelial cell adhesive interactions (LECA) and venular protein leakage (VPL) in single postcapillary venules of the rat mesentery. The effects of bradykinin superfusion to increase LECA and VPL were prevented by coincident topical application of either a bradykinin-B(2) receptor anta...

Hereditary angioedema (HAE) causes recurrent episodes of angioedema that may be very severe and are frequently associated with significant morbidity and even mortality. Understanding the pathophysiology of this disease is crucial for proper diagnosis and management of these patients. HAE is caused by mutations in the SERPING1 gene that result in decreased plasma levels of functional C1 inhibito...

Bereits der Zusatz des durch bloßes Abspülen der Tiere mit Methanol gewonnenen Schleimextraktes an den isolierten Rattenuterus und das Meerschweinchenileum löste an diesen Organen Kontraktionen aus. Dieser stimulierende Effekt blieb auch nach Zugabe der Antagonisten gegenüber den üblichen in Organextrakten gelegentlich vorkommenden biologisch aktiven Begleitsubstanzen voll erhalten. Da die Wirk...