Yuh-Nung Jan is at the University of California, San Francisco (UCSF). He studied Physics as an undergraduate at National Taiwan University. In 1968, he entered Caltech as a graduate student in theoretical high-energy physics. Two years later, he switched to biology. After he completed his Ph.D. with Max Delbrück in 1974, he joined Seymour Benzer’s lab as a postdoctoral fellow. There he began w...

dILA and dILB neurons comprise the major neuronal subtypes generated in the dorsal spinal cord, and arise in a salt-and-pepper pattern from a broad progenitor domain that expresses the bHLH factor Mash1. In this domain, Mash1-positive and Mash1-negative cells intermingle. Using a Mash1(GFP) allele in mice, we show here that Mash1+ progenitors give rise to dILA and dILB neurons. Using retroviral...

Asymmetric cell division (ACD) is a simple and evolutionary conserved process whereby a mother divides to generate two daughter cells with distinct developmental potentials. This process can generate cell fate diversity during development. Fate asymmetry may result from the unequal segregation of molecules and/or organelles between the two daughter cells. Here, I will review how fate asymmetry ...

The primary root of Arabidopsis has a simple cellular organisation. The fixed radial cell pattern results from stereotypical cell divisions that occur in the meristem. Here we describe the characterisation of schizoriza (scz), a mutant with defective radial patterning. In scz mutants, the subepidermal layer (ground tissue) develops root hairs. Root hairs normally only form on epidermal cells of...

C lassic experiments in C. elegans showed that a neuroblast stem cell, called the Q cell, undergoes sequential asymmetric cell divisions that result in daughter cells with dramatically different cell fates. The daughters of the fi rst division migrate to different locations in the animal where they divide again. A further round of division generates a small daughter cell that immediately dies b...

Both Drosophila neuroblasts and Caenorhabditis elegans zygotes use a conserved protein complex to establish cell polarity and regulate spindle orientation. Mammalian epithelia also use this complex to regulate apical/basal polarity. Recent results have allowed us to compare the mechanisms regulating asymmetric cell division in Drosophila neuroblasts and the C. elegans zygote.

Caenorhabditis elegans embryos establish cortical domains of PAR proteins of reproducible size before asymmetric cell division. The ways in which the size of these domains is set remain unknown. Here we identify the GTPase-activating proteins (GAPs) RGA-3 and RGA-4, which regulate the activity of the small GTPase RHO-1. rga-3/4(RNAi) embryos have a hypercontractile cortex, and the initial relat...

Growth in biofilms provides bacterial species with many advantages over growth in suspension, e.g. colonization of nutrient-rich areas. In the α-proteobacterium Caulobacter crescentus biofilm formation is facilitated through its asymmetric cell division, where one daughter cell becomes a motile flagellated swarmer cell able to colonize new surfaces while the other remains as a stalked cell atta...

During Drosophila neurogenesis, glial differentiation depends on the expression of glial cells missing (gcm). Understanding how glial fate is achieved thus requires knowledge of the temporal and spatial control mechanisms directing gcm expression. A recent report showed that in the adult bristle lineage, gcm expression is negatively regulated by Notch signaling ( Van De Bor, V. and Giangrande, ...

Caulobacter crescentus divides asymmetrically and creates distinct polar membrane surfaces that partition during the cell cycle to distinct cell progeny. Blocking membrane synthesis prevented transcription from selective promoters involved in asymmetric cell division. Transcription from sigma-54-dependent flagellar promoters was blocked completely; however, transcription from the CtrA response ...