Alkaline Protease enzyme produced from Bacillus Licheniformis PTCC1331 in optimum Condition(Temperature 37 ?C, 150 rpm, PH=10), is immobilized in different concentrations of Sodium Alginate using entrapment method, to increase its thermal resistance in detergents, and its activity is measured thereafter. Maximum enzymatic activity of 46 was obtained in 2%w/v Sodium alginate solution. Investiga...

Glucose 6-phosphate dehydrogenase (G6PD) from Streptomyces aureofaciens was purified and denatured in 6 M urea. Denaturation led to complete dissociation of the enzyme into its inactive monomers, 98% loss of the enzyme activity, about 30% decrease in the protein fluorescence and a 10 nm red shift in the emission maximum. Dilution of urea-denatured enzyme resulted in regaining of the enzyme acti...

conclusions: it was concluded that the elisa is a suitable method for large scale screening of antibodies against aspergilloma. the easy techniques have shortened the required time of tests (approximately 1.30 hours for our elisa, as opposed to 2.30 hours with commercial elisa kit). this may be a substantial benefit for large numbers of samples that should be tested. materials and methods: an i...

the protease enzyme breaks down protein into amino acids as its constructors. protease immobilization onto appropriate support materials plays an essential role in various fields of technology including the food and detergent industries. accordingly, improvement of protease immobilization has been highly regarded due to its applications in bio catalysis. alginate and chitosan are natural polysa...

background: nuts are one of the main consumed snacks worldwide and also have an important role among iranian's food habits. natural contamination of nuts with aflatoxin is unavoidable and causes a special challenge for nuts safety and quality. objectives: the purpose of this research was to study the aflatoxin contamination in commercially-available nuts (pistachio, walnut and peanut) in the ma...

the objectives of this study were to determinethe interactions between two hepatic enzymes and some minerals in the liver of broiler chickens. the study was performed with male and female from 1 to 56 days of age of broiler chickens. malic acid was added to the water and offered to chickens freely from the start to the end of the experiment with constant concentration. the treatments consisted ...