نتایج جستجو برای: agroinfiltration
تعداد نتایج: 171 فیلتر نتایج به سال:
Our previous results demonstrated that the DNAbeta satellite (Y10beta) associated with Tomato yellow leaf curl China virus Y10 isolate (TYLCCNV-Y10) is essential for induction of leaf curl symptoms in plants and that transgenic expression of its betaC1 gene in Nicotiana plants induces virus-like symptoms. In the present study, in vitro DNA binding activity of the betaC1 proteins of Y10beta and ...
A transcriptomic approach has been used to identify genes predominantly expressed in maize (Zea mays) scutellum during maturation. One of the identified genes is oil body associated protein1 (obap1), which is transcribed during seed maturation predominantly in the scutellum, and its expression decreases rapidly after germination. Proteins similar to OBAP1 are present in all plants, including pr...
BACKGROUND Plant lipoxygenases (LOXs) have been proposed to form biologically active compounds both during normal developmental stages such as germination or growth as well as during responses to environmental stress such as wounding or pathogen attack. In our previous study, we found that enzyme activity of endogenous 9-LOX in Nicotiana benthamiana was highly induced by agroinfiltration using ...
[This corrects the article on p. 1171 in vol. 7, PMID: 27555856.].
The ubiquitin/26S proteasome system plays an essential role not only in maintaining protein turnover, but also in regulating many other plant responses, including plant-pathogen interactions. Previous studies highlighted different roles of the 20S proteasome in plant defense during virus infection, either indirectly through viral suppressor-mediated degradation of Argonaute proteins, affecting ...
BACKGROUND Codon optimization and subcellular targeting were studied with the aim to increase the expression levels of the SAG178-322 antigen of Toxoplasma gondii in tobacco leaves. The expression of the tobacco-optimized and native versions of the SAG1 gene was explored by transient expression from the Agrobacterium tumefaciens binary expression vector, which allows targeting the recombinant p...
The potato cyst nematode Globodera rostochiensis invades roots of host plants where it transforms cells near the vascular cylinder into a permanent feeding site. The host cell modifications are most likely induced by a complex mixture of proteins in the stylet secretions of the nematodes. Resistance to nematodes conferred by nucleotide-binding-leucine-rich repeat (NB-LRR) proteins usually resul...
vp2 protein is the major host protective immunogen of infectious bursal disease virus (ibdv) of chickens. plants are now gaining widespread acceptance as a general platform for the large scale production of recombinant proteins including vaccine, antibodies and pharmaceuticals. in present study vp2 gene of an iranian isolate of ibdv was cloned under strong plant expression promoter camv35s and ...
Introduction: Human growth hormone (HGH) is very important pharmaceutical substance which is used as an absolute cure for different diseases that is why the production of HGH recombinant is taken into consideration not only nationally but also internationally. Many different methods and host systems have been used as growth hormone factories. Finding new and efficient hosts for recombinant mole...
تراریختی گذرای گیاهان روشی مفید و در برخی موارد جایگزینی مناسب برای تراریختی پایدار به ویژه در غلات، گیاهان درختی و گیاهانی است که باززایی و یا تراریختی آنها با مشکل مواجه است. در این روش در زمانی کوتاه تعداد زیادی از نسخههای تراژن وارد سلول گیاهی شده، رونویسی و ترجمه میشوند و چون توالی DNA در ژنوم میزبان وارد نمیشود، بیان ژن تحت تاثیر محل درج و تغییرات اپیژنتیکی قرار نمیگیرد. تراریختی گذرا...
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