Error-prone PCR was used to isolate Photinus pyralis luciferase mutants producing bright light in the red-orange region of the spectrum. All mutations were clustered in the beta5-alpha10-beta6 region of N-terminal subdomain B and appear to affect bioluminescence color by modulating the position of the Ser314-Leu319 mobile loop with respect to the putative active site. Two red variants (Q283R an...

Common mycorrhizal network (CMN) allows nutrients and signals to pass between two or more plants. In this study, trifoliate orange (Poncirus trifoliata) and white clover (Trifolium repens) were planted in a two-compartmented rootbox, separated by a 37-μm nylon mesh and then inoculated with an arbuscular mycorrhizal fungus (AMF), Diversispora spurca. Inoculation with D. spurca resulted in format...

OBJECTIVE To measure iron absorption in children from meals containing apple juice or orange juice so as to determine if iron absorption will be greater with orange juice because of its higher ascorbic acid content than apple juice, a noncitrus fruit juice that US children reportedly prefer. DESIGN On 2 successive days, children consumed identical meals that included apple juice on one day an...

Production of intracellular β-xylosidase was studied in cultures of Penicillium janthinellum grown on citrus fruit waste supplemented cultivation media. Both dried orange peel and sweet lime peel could induce the production of this enzyme. The working strain showed a pronounced optimum pH and temperature for β-xylosidase production at 6.0 and 27 °C, respectively. The enzyme production was found...

We studied the effects of cooking on the vitamin and mineral content of vegetables (vegetable soup, cauliflower), meat (beefsteak) and fish (sole) and those of cutting (fruit salad) and squeezing (orange juice) on the vitamin content of fruits. In cooked dishes, vitamin retention ranged between 0 (folic acid, all dishes) and 94% (retinol, sole) and mineral retention between 63 (copper, cauliflo...

This paper describe the high-voltage pulsed discharge generated in water with different types of bubbling gases as well as no gas in a reactor with a point-mesh electrode configuration. The pulsed discharges are a promising technique in environmental remediation to treatment of wastewater. Acid orange 7organic dye solution was treated as a common wastewater sample to elucidate chemical efficien...

Electrical heating of food products provides rapid and uniform heating, resulting in less thermal damage to the product. The objective of this research was to examine the effects of ohmic heating on the stability of orange juice with comparison to conventional pasteurization. During storage at 4 degrees C, degradation curves of ascorbic acid followed a linear decrease pattern in both ohmic-heat...

W. Barros-Parada, A.L. Knight, E. Basoalto, and E. Fuentes-Contreras. 2013. An evaluation of orange and clear traps with pear ester to monitor codling moth (Lepidoptera: Tortricidae) in apple orchards. Cien. Inv. Agr. 40(2):307-315. Studies were conducted to evaluate the use of several trap-lure combinations to improve the monitoring of codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricid...

Ascorbic acid contents of some fruits and vegetables in Abakaliki were analyzed through titrimetric methods. The results recorded the concentrations (mg/100g) of ascorbic acids in various fruits as orange (15.47 ± 0.23), lemon (12.87 ± 0.45), grape (2.00 ± 0.10), paw-paw (46.40 ± 1.65), apple (2.20 ± 0.10), banana (1.87 ± 0.06), carrot (1.80 ± 0.04), pineapple (2.80 ± 0.10), water melon (2.27 ±...