Fifty-two ovine strains of Pasteurella haemolytica and P. trehalosi representing serotypes 1-16 were examined for the presence of [copper, zinc]superoxide dismutase DNA sequences. This was done using a combination of polymerase chain reaction with degenerate primers based on the sequence of the [Cu,Zn]superoxide dismutase gene (sodC) in related species and Southern hybridization using a fragmen...

Mutations in the gene encoding Cu/Zn superoxide dismutase-1 cause amyotrophic lateral sclerosis. Superoxide dismutase-1 mutations decrease protein stability and promote aggregation. The mutant monomer is thought to be an intermediate in the pathway from the superoxide dismutase-1 dimer to aggregate. Here we find that the monomeric copper-apo, zinc-holo protein is structurally perturbed and the ...

background: despite the genetic heterogeneity reported in familial als (fals), sod1 gene mutations are the most frequent cause of fals, accounting for around 20% of familial cases (als1) and isolated sporadic cases. mutant forms of sod1 exhibit toxicity that promotes the death of motor neurons. it is well documented that fals produces protein aggregates in the motor neurons of fals patients, wh...

Burn injury is a medical problem as well as a social burden on the national health services in developing countries. Trace elements have important roles in wound healing and act as antioxidants. In this study we estimated iron (Fe), zinc (Zn) and copper (Cu) levels in serum and superoxide dismutase and catalase activities in erythrocyte of burned patients. Study includes 43 patients with burn i...

The present work was undertaken to study the effects of 28-homobrassinolide on growth, zinc metal uptake, antioxidative enzyme [guaiacol peroxidase (EC 1.11.1.7), catalase (EC 1.11.1.6), glutathione reductase (EC 1.6.4.2), ascorbate peroxidase (EC 1.11.1.11), superoxide dismutase (EC 1.15.1.1)] activities and protein content in 7-d-old seedlings of Brassica juncea L. treated with Zn metal at di...

Four rice genotypes differing in Zn efficiency were selected to study the physiological mechanisms for different susceptibility of genotypes to Zn deficiency. It was demonstrated that Zn efficiency was not associated with flooding tolerance, so that flooding tolerance trait could be detected among both Zn-efficient and Zn-inefficient genotypes. Total and water soluble Zn concentrations in shoot...

The iron containing superoxide dismutase from E. coli B is shown to catalyze superoxide dismutation by a mechanism which exhibits saturation kinetics. This behavior is quite different from that observed previously with bovine Zn/Cuand iron-containing superoxide dismutase from P. leiosnathi. Two parameters of catalysis were measured in the pH range 7.2 to 10.4: k was found to be independent of p...

Skeletal muscle contractions increase superoxide anion in skeletal muscle extracellular space. We tested the hypotheses that 1) after an isometric contraction protocol, xanthine oxidase (XO) activity is a source of superoxide anion in the extracellular space of skeletal muscle and 2) the increase in XO-derived extracellular superoxide anion during contractions affects skeletal muscle contractil...