In the voltage-sensitive phosphatase Ci-VSP, conformational changes in the transmembrane voltage sensor domain (VSD) are transduced to the intracellular catalytic domain (CD) leading to its dephosphorylation activity against membrane-embedded phosphoinositides. The linker between both domains is proposed to be crucial for the VSD-CD coupling. With a combined approach of electrophysiological mea...

To identify and provide a global assessment of DNA methylation in fetal ventricular septal defect (VSD), genomic DNA extracted from fetal myocardial tissue samples with VSD (n=21) and from normal fetal myocardial tissue samples (n=15) was analyzed for gene methylation using array‑based technology. Furthermore, the KIAA0310, RAB43, SIVA1 and NDRG2 genes were randomly selected for validation anal...

Allostery provides a critical control over enzyme activity, biasing the catalytic site between inactive and active states. We found that the Ciona intestinalis voltage-sensing phosphatase (Ci-VSP), which modifies phosphoinositide signaling lipids (PIPs), has not one but two sequential active states with distinct substrate specificities, whose occupancy is allosterically controlled by sequential...

4 Introduction 5 Methods 8 Structure 8 Elastic Network Models 9 Evolutionary Conservation 9 Results 11 a. hinges and rigid elements 11 GNM 11 ANM 13 b. The correlation between the fluctuations 13 Identification of motions in groups of slow modes 13 The PD 16 The motion of the VSD 17 I. The hinge between the domains 17 II. The S4 helix 18 III. Other hinges in the VSD 19 Independent motions of th...

Kidney transplantation from older and marginal donors is effective to confront organ shortage. However, limitations after of kidneys very kidney remain unclear. We compared patient graft outcome, achieved allograft function quality life renal transplantations Very Senior Donors (VSD, defined as aged 70 years older) with (SD, 60-70 years) Regular (RD, younger than 60 years) in Switzerland. evalu...

Acquired intracardiac left-to-right shunts are rare occurrences. Chest trauma and myocardial infection are well-known causes of acquired ventricular septal defect (VSD). There have been several case reports describing left ventricle to right atrium shunt after infective endocarditis (IE). We present here a patient found to have an acquired VSD secondary to IE of the aortic and tricuspid valves ...

Figure 1 Transesophageal echocardiogram (TEE). Two-dimensional TEE without (A) and with color (B) showing subarterial VSD (red arrow) and hypertrophy of the basal septum (white arrow) below the VSD. The defect was w5 mm in size and hemodynamically benign as the pulmonary to systematic blood flow ratio (Qp/Qs) was calculated from cardiac catheterization data to be 1.6:1. The ascending aorta was ...

The case of a 20 years old male, diagnosed as supracristal ventricular septal defect (VSD) for last 6 years is being presented. He came in emergency department with decompensated congestive cardiac failure. After initial stabilization, he underwent transthoracic echocardiogram which showed large supracristal VSD, severely prolapsing right coronary cusp, severe aortic regurgitation and severe pu...

Background/Aim: Ventricular septal defect (VSD) is the most common congenital heart anomaly that in many cases closes spontaneously. The spontaneous closure (SC) rate of VSD varies widely between studies. aim this study was to identify clinical and echocardiographic factors influencing SC isolated first year life among a group patients presented at Paediatric Clinic. Methods: Prospective perfor...