The polymerase chain reaction (PCR) is used widely for the in vitro amplification of DNA. The versatility of PCR can be enhanced by the introduction of sequential steps such as nested primer amplifications (1) or re-amplification protocols (2). PCR can also be done on cDNA templates produced by reverse transcription of RNA (3). These protocols require additional opening and closing of the react...

Sequence analysis of the ORFK1 human herpesvirus type 8 (HHV-8) allows identification six major subtypes (A–F), which are related to migrations and clinical progression Kaposi's sarcoma. Sequencing subsequent phylogenetic is considered be most reliable method for HHV-8 genotyping. However, it exhibits challenges limitations. Herein, we designed validated a single base extension (SBE) protocol c...

A nested PCR assay to detect parasite DNA in slit aspirates from skin lesions of patients with post-kala-azar dermal lesihmaniasis (PKDL) is described. PCR results were positive in 27 of 29 (93%) samples by nested PCR assay, while only 20 of 29 (69%) were positive in a primary PCR assay. The nested PCR assay allowed reliable diagnosis of PKDL in a noninvasive manner.

BACKGROUND & OBJECTIVE Glycoprotein B (gB), involved in cell-to-cell transmission of human cytomegalovirus (HCMV), is a critical factor in tissue tropism and viral pathogenesis. The aim of the present study was to compare the efficiency of PCR-based RFLP and multiplex nested PCR for gB gene of HCMV to determine their genotype in clinical specimens from patients with HCMV. METHODS The PCR base...