A simple procedure for preparation of an affinity resin with 3'-amino thymidine linked to the carboxyl residues on 6-amino-hexanoic agarose is described. We have used this column for a rapid and simple purification of the thymidine kinase encoded by the herpes simplex virus type 1 genome. This resin has two major advantages over the most widely use used resin made with thymidine-p-nitrophenyl p...

Commercial [methyl-3H]thymidine preparations tested here contain about a 0.2% contaminant which is rapidly incorporated into Chlamydomonas DNA. This contaminant obscures the measurement of the rate of chloroplast DNA synthesis when methyl-labeled preparations are used. Such contaminants are not present in ring-labeled (either 3H or 14C) thymidine preparations. In ring-labeled thymidine preparat...

The inhibitory activity of liver extracts on thymidine incorporation into DNA was markedly enhanced by manganese and zinc. Manganese-activated liver extract (Mn-liver extract) also inhibited incorporation of deoxycytidine and adenine into DNA. The Mn-liver extract had no effect on incorporation of thymidine-5'-triphosphate into DNA but blocked the phosphorylation of deoxythymidine. The phosphor...

Thymidine kinase 1 (TK 1-fetal) is a cell cycle-dependent marker that increases dramatically during the S-phase of the cell cycle. In this review, the authors discuss serum levels of thymidine kinase in a variety of neoplasias. Determination of thymidine kinase helps to monitor the follow-up of solid tumours and haematological malignancies as well as indicating the efficacy of adjuvant and pall...

In heterokaryons between senescent and young diploid fibroblast-like cells, dominance of the former with respect to nuclear DNA synthesis (incorporation of [(3)H]thymidine) was demonstrated. For identification of the respective partners, double-layer autoradiography was used after the old cells were labeled with [(3)H]methionine and the young cells were labeled with [(14)C]thymidine. Synchrony ...

The uptake of thymidine into an acid-soluble pool in normal hamster embryo cells and hamster tumor cells transformed by polyoma virus was studied. It was found that tumor cells exhibited a 4-fold higher capacity than normal cells to incorporate thymidine as phosphorylated products into this pool. This was correlated with a 5to 10-fold higher activity of thymidine kinase in the tumor cell lines....

Partially purified samples of thymidine phosphorylase were obtained from four preparations of human uterine leiomyomas and uteri using the method of Yoshimura et al. (1990), Biochim. Biophys. Acta 1034, 107-113. Among the studied twelve pyrimidine derivatives, 5-bromouracil, 5-nitrouracil, 5-fluorouracil, 6-aminouracil, 4, 6-dihydroxy-5-nitropyrimidine are competitive inhibitors, while allyloxy...

The use of a thymidine block' for the induction of synchronous cell division in lymphocyte cultures is gaining in popularity in clinical cytogenetics. The excess thymidine, converted to thymidine triphosphate within the cell, inhibits the ribonucleotide reductase catalysed reduction of cytidine diphosphate to deoxycytidine diphosphate. This leads to a depleted deoxycytidine 5'-triphosphate pool...

Thymidine autoradiography and retrograde transport of horseradish peroxidase (HRP) were combined to determine the connectivity of neurons born in adult canary forebrain. Adult male and female canaries were pretreated with [3H]thymidine to label cells undergoing DNA synthesis prior to mitosis. Thirty or 60 days later, neurons in a forebrain nucleus, hyperstriatium ventralis, pars caudalis (HVc),...

Boron neutron capture therapy (BNCT) is a chemoradio-therapeutic method for the treatment of cancer. It depends on the selective targeting of tumor cells by boron-containing compounds. One category of BNCT agents with potential to selectively target tumor cells may be thymidine derivatives substituted at the 3'-position with appropriate boron moieties. Thus, several thymidine analogues were syn...