Mast cell colonies grew in methylcellulose when mouse spleen cells were cultured for 14 days in the presence of medium conditioned by concanavalin A-stimulated spleen cells. Tumor-promoting phorbol esters, 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12,13-didecanoate, enhanced the concanavalin A-stimulated spleen cell-conditioned medium-induced colony growth of mast cell progenitors....

This new assay of viral cell-mediated immunity is sensitive, reproducible, and in many ways resembles the in vivo state. The spread of herpes simplex virus between adjacent monolayer cells was inhibited in the presence of spleen cells from guinea pigs sensitized to that virus. This in vitro control of viral growth was quantified by determining plaque size in monolayers to which were added sensi...

Mouse marrow cells were exposed to 5-fluorouracil (FU) either in vivo or in vitro and the effects on the hematopoietic stem cell compartment were studied. The drug was highly toxic to bone marrow cells including the spleen colony-forming unit (CFU-S) population. The small population of stem cells surviving FU, however, caused a different pattern of spleen colony growth when injected into lethal...

Abstract Multiple sclerosis (MS) is caused by a T cell orchestrated immune response to myelin sheath antigens of the central nervous system. Autoimmune responses can be suppressed CD4 +Foxp3 +T regulatory cells (Treg); therefore, therapeutic strategy increase endogenous Treg numbers. Our studies using mouse model MS, experimental autoimmune encephalomyelitis, have identified unique subset IgM +...

BACKGROUND Spleen as a lymphoid tissue is specialized for monitoring blood and mounting immunity against blood-borne antigens. Antigen-presenting cells present in spleen commonly develop from bone marrow-derived precursors that enter blood circulation. However, a distinct splenic myeloid antigen-presenting cell subset described in this laboratory, namely "dendritic-like cells" (L-DC), has been ...

The present study concerns the isolation, characterization, origin, and kinetics of spleen macrophages. The spleen was first perfused in situ to remove monocytes from the vascular bed and then dissected and treated with collagenase. The macrophages in the cell suspension thus obtained were characterized morphologically and cytochemically and then quantitated. The spleen cell suspension was incu...

The effect of preincubation with anti-theta or anti-mouse immunoglobulin (Ig) and complement (C') on immune responsiveness of spleen cells from BALB/c mice immunized with sheep erythrocytes (SE) was investigated. Both treatments greatly depressed the remaining ability to produce a secondary response to SE in vitro. Normal BALB/c spleen cells were far less effective in reconstituting the respons...

Persistent infection was established in SCID mice given 10(7) Cryptosporidium parvum oocysts. Nine groups of infected SCID mice were inoculated with 10(6), 10(5), or 10(4) total spleen cells, CD8-depleted spleen cells, or CD4-depleted spleen cells from naive BALB/c donors. Infection was significantly reduced in all treatment groups. The most profound effect occurred with spleen cell preparation...

Abstract Pancreatic ductal adenocarcinoma (PDA) is a lethal malignancy with poor response to conventional immune checkpoint inhibitors. Early data by our group suggests CD4 T cell depletion abrogates novel combination therapy that transiently curative in murine model of PDA. Given the importance cells PDA control, we now seek describe dynamics at an antigen-specific resolution. To this end, dev...

To study graft-versus-host reaction (GVHR) in small-bowel transplantation and its underlying mechanisms and to find methods for circumventing GVHR, we used an unidirectional GVHR model in which F1 Lewis (LEW) x Wistar King A (WKA) hybrid rats received small-bowel transplants from either LEW or WKA parent rats. The survival time of F1 hybrid rats that received full-length small-bowel transplanta...