A UV-spectrophotometric method is described for the determination of lansoprazole (LAN). The method is based on the measurement of the absorbance of LAN solution in acetonitrile at 281 nm. The system obeyed Beer’s law over the concentration range of 1.2525.0 μg/mL. The degradation behavior of LAN was investigated under dry heat treatment, UV-degradation, acid hydrolysis, alkali hydrolysis and o...

The H-point standard addition method (HPSAM), based on a spectrophotometric measurement for the simultaneous determination of hydrazine and acetylhydrazine, is described. This method is based on the difference between the rates of their reactions with N,N-dimethylaminobenzaldehyde (DAB) in the presence of sodium dodecyl sulfate (SDS) in acidic media. The results showed that hydrazine and acetyl...

A diverse collection of actinomycete strains were screened for production of extracellular peroxidase activity by adapting a chemiluminescence analysis system developed for horseradish peroxidase-based enzyme-linked immunosorbent assay. Extracellular peroxidase activity was found to be common but quantitatively variable, and this rapid and sensitive screening system permitted identification of ...

A flow-injection method has been developed for the sequential spectrophotometric determination of iron and titanium using 3,4 dihydroxybenzoic acid as chromogenic reagent. The system involves the sequential measurement of the absorbances of the complexes at 380 and 570 nm. The system is designed using a simultaneous injection of sample and reagent into separate carrier streams. The proposed met...

Van Niel's classical distinction between bacterial and green plant photosyntheses is based on the differences in the metabolism of the photooxidant (16). But direct measurement of the oxidizing power in bacterial photosynthesis is, at best, difficult with the usual spectrophotometric equipment. Light-induced oxidations of endogenous cytochromes have been demonstrated in whole cells and extracts...

Ireport a detailed series of microcalorimetric measurement of glucose concentrations in five reference samples of serum. The method utilized (a) calibration in the actual medium of analysis and (b) a correction for interferences owing to the nonspecificity of the enzyme hexokinase. The microcalorimetric results are compared with the tentative results of analyses obtained by the spectrophotometr...

Three simple, rapid, accurate, precise and cost-effective UV spectrophotometric methods have been developed and validated for simultaneous estimation of Escitalopram oxalate and Etizolam in tablet dosage form. The UV methods have been developed utilizing concept of standard addition utilizing 0.1 N HCl as a solvent. Method I is estimation using simultaneous equation method at 238.2 nm (λmax of ...

A spectrophotometric method has been developed for the rapid measurement of the antimicrobial activity of natural products, including crude extracts or pure materials. The assay depends on the measurement of non-specific esterase activity using fluorescein diacetate (FDA) hydrolysis in broth cultures of microbes after they have been treated with test compounds. The assay is accurate, reproducib...

the purpose of this investigation was to determine the antioxidant potentials and anti-glucosidase activities of six tropical medicinal plants. the levels of phenolic constituents in these medicinal plants were also quantified and compared. antioxidation potentials were determined colorimetrically for scavenging activities against dpph and no radicals. metal chelating assay was based on the mea...

Phenolic compounds are intermediary transformation products of many naturally occurring substances and synthetic chemicals, including crop residues, organic wastes, pesticides and industrial products. They are suitable substrates for the phenolases and peroxidases produced by the soil microflora. Although measurement of the enzymatic oxidation of phenolic compounds is an important measure of th...