Background and Aims: Coated nanoparticles have different surface chemistry, aggregation, and interaction properties. The aim of this study was to investigate the cytotoxicity of silver nanoparticles AgNPs coated with different proteins on Balb/c macrophages. Materials and Methods: In this study these items were evaluated: 1) the size of aggregation, 2) the quantity and mechanisms of uptake, ...

BACKGROUND Gc protein has been reported to be a precursor of Gc protein-derived macrophage activation factor (GcMAF) in the inflammation-primed macrophage activation cascade. An inducible beta-galactosidase of B cells and neuraminidase of T cells convert Gc protein to GcMAF. MATERIALS AND METHODS Gc protein from human serum was purified using 25(OH)D3 affinity column chromatography and modifi...

PURPOSE To study the glycoconjugates in the lacrimal sac epithelium of Japanese white rabbits with experimentally induced chronic dacryocystitis. METHODS Chronic dacryocystitis was induced by a subcutaneous injection of albumin followed by an injection of Staphylococcus aureus into the lacrimal sac. The histological appearance of the lacrimal sac was studied using the alcian blue-periodic aci...

We constructed an arming (cell-surface-engineered) yeast displaying two types of agglutinin (modified a-agglutinin and alpha-agglutinin) on the cell surface, with agglutination being independent of both mating type and pheromones. The modified a-agglutinin was artificially prepared by the fusion of the genes encoding Aga1p and Aga2p. The modified a-agglutinin could induce agglutination of cells...

The heat-labile sexual agglutinin from Saccharomyces kluyveri strain 17 (alpha-agglutinin) has been isolated in an apparently intact form from wild-type and mnn1 (glycosylation-defective) cells. The wild-type agglutinin is polydisperse, due to variable degrees of glycosylation, and migrates on native gels with an apparent mass of greater than 400 kDa, whereas under denaturing conditions it appe...

A naturally occurring hemagglutinin (HA), with activity against bacteria and yeast cells were detected in the serum of Scylla serrata using mammalian erythrocytes (RBC), various bacteria and yeast as indicator cells. The serum gave highest HA titer with rabbit RBC, tripsinized yeast and Vibrio fluvialis. An analysis of the physico-chemical properties of the HA showed it to be specifically depen...

Introduction Peach allergy is one of the most common allergies in subjects with reactions to plant-foods. The relationship between peach allergy and other foods and the sensitization pattern in these patients have not been studied in detail. We study the sensitization to relevant allergens, such as peach and peanut LTP, Prup3 and Arah9, and seed storage protein, Arah2. Studies were carried out ...