Cleavage of gentiobiose in cell extracts of gentiobiose-grown Aerobacter aerogenes was dependent on the presence of adenosine 5'-triphosphate (ATP). The enzymes that participate in the overall reaction were shown to be a beta-glucoside kinase, which catalyzes the phosphorylation of gentiobiose with ATP to form gentiobiose monophosphate [6-O-phosphoryl-beta-d-glucopyranosyl-(1 --> 6)-d-glucose],...

The Ancient Egyptians recognized the pain-relieving effects of concoctions made from myrtle and willow leaves. Hippocrates may have been among the first to use willow bark and leaves from the Salix species to relieve fever, but it was not until 1829 that the glycoside salicin was extracted from the willow bark and used as an antipyretic. Seven years later, salicylic acid was isolated, and by th...

A CULTURE of a new Salmonella type of organism was received during August, 1952; it had been isolated from several patients suffering from food poisoning at Seremban, Malaya (Robinson, T. M., 1952, personal communication). On desoxycholate-citrate agar this organism produced non-lactose fermenting colonies typical of the Salmonella group. It is a Gram-negative, motile rod with the following bio...

Nineteen strains of Gram-positive cocci isolated from wine and belonging to the genus Leuconostoc were examined and are considered to form a new species which is named Leuconostoc oenos. These strains differ from other leuconostocs because they grow better in a medium containing tomato juice than in yeast glucose citrate broth normally used in this laboratory for the genus. Furthermore, they gr...

A high performance liquid chromatography method was established for simultaneously determining four bioactive components, salicin, liquiritin, paeonolum, and imperatorin in Fengshiding capsule, a widely used traditional Chinese medicine for treating rheumatic disease. The chromatographic separation was performed on a Shimadzu Shim-pack Stable Bond C18 column using gradient elution with methanol...

A modified version of sorbitol MacConkey medium containing cefixime and tellurite (CT-SMAC medium) was produced by adding salicin and 4-methylumbelliferyl-beta-D-galactopyranoside to CT-SMAC medium; this medium was designated CT-SSMAC medium and was used to isolate Escherichia coli O157:H7 from radish sprouts. Of 101 non-E. coli bacteria isolated from radish sprouts that produced colorless colo...

A new serotype, Salmonella alachua,2 is represented by one culture isolated during a study of salmonellosis in swine in Florida. The culture was isolated from a sample of soil taken from a pen or holding lot in which swine were kept prior to slaughter. The soil was heavily contaminated with feces. The sample was collected by inserting a sterile dry swab into the moist ground and then placing th...

The utilization of various carbohydrates by species of streptomyces has been suggested by Pridham and Gottlieb (1948), Kurosawa (1951), and Benedict et al. (1955) as an aid in differentiation of species. While the latter investigators have reported that streptomycin-producing strains of Streptomyces griseus will grow on synthetic agar media containing as sole carbon source mannitol, xylose, fru...