the conditional formal potential, e°′of methyldopa has been studied by cyclic voltammetry at the surface of activated glassy carbon electrode (agce) as the working electrode in different ph phosphate buffered solutions. the experimental standard redox potential, e°′, of methyldopa is obtained to be 0.72 mv versus she (standard hydrogen electrode). e°′ values have also been calculated with the a...

Etoposide (VP-16) is extensively used to treat cancer, yet its efficacy is calamitously associated with an increased risk of secondary acute myelogenous leukemia. The mechanisms for the extremely high susceptibility of myeloid stem cells to the leukemogenic effects of etoposide have not been elucidated. We propose a mechanism to account for the etoposide-induced secondary acute myelogenous leuk...

The redox behavior cobalt chloride was studied voltammetrically in presence and absence of L- Carrageenan (LK) natural polymer using glassy carbon electrodes in 0.1 M KCl supporting electrode. Scan rates are studied for the redox behaviors for CoCl2 alone or in presence of L- Carrageenan (LK) natural polymer. Stability constants for the interaction of cobalt ions with L - Carageenan (LK) natura...

Iron(II) in buffer medium and in presence of oxalate has been proposed as a new electron transfer reagent or reducing agent for the selective determination of chromium(VI) in presence of several other metal ions such as Mo(VI), U(VI), V(IV) etc. The method consists in taking a chromium(VI) solution [ containing 3-11mg of Cr] into a buffer medium of pH ~ 4.05.2 containing about 0.040.10M potassi...

deficiency. It is not applicable to newborn or mass screening with use of ifiterpaper specimens, but is satisfactory for liquid urine screening. In one variant of sulfite oxidase deficiency, due to a defect of its molybdate cofactor metabolism, the concentration of uric acid in serum is decreased. Thus, an unexplained low serum uric acid, especially in a young patient with neurologic abnormalit...

Hydrogen peroxide emerged as major redox metabolite operative in redox sensing, signaling and redox regulation. Generation, transport and capture of H2O2 in biological settings as well as their biological consequences can now be addressed. The present overview focuses on recent progress on metabolic sources and sinks of H2O2 and on the role of H2O2 in redox signaling under physiological conditi...

Recombination systems based on lambda and Cre/loxP have been described to facilitate gene transfer from one vector to another in a high-throughput fashion, avoiding the bottlenecks associated with traditional cloning. However, no system described to date is suitable for the cloning of affinity reagents selected from display libraries, which requires that the recombination signals flanking the a...

We developed a new approach for chemical ligation of oligonucleotides using the electrophilic phosphorothioester (EPT) group. A nucleophilic phosphorothioate group on oligonucleotides was converted into the EPT group by treatment with Sanger's reagent (1-fluoro-2,4-dinitrobenzene). EPT oligonucleotides can be isolated, stored frozen, and used for the ligation reaction. The reaction of the EPT o...