In this study, our previously reported novel synthetic gene encoding 166 residues of interferon-? was used for an efficient expression of IFN-?. The synthetic gene was cloned into pET21a expression vector and transferred into E. coli. Recombinant protein was over-expressed in the E. coli. Identity of the recombinant protein was confirmed by western blot analysis. The recombinant protein was bio...

diphtheria is a fatal disease caused by exotoxin of corynebacterium     diphtheria .  this toxin consists of   two chains, catalytic chain (a) and binding (b) chain. by binding chain (b),   the toxin binds to its receptor on numerous body cells such as myocardial,   kidney and peripheral nerve cells. after entering, catalytic chain (a)   inhibits protein synthesis and finally can cause cell dea...

b ackground: the prevalence of urinary tract infection (uti) is really high in the world. escherichia coli is a major agent of uti. one of the strategies for decreasing uti infections is vaccine development. as the attachment is a really important stage in colonization and infection, at- tachment inhibition has an applied strategy.  fimh protein is a major factor during bacterial colonization i...

Immunization with DNA can induce humoral and cell-mediated immune responses, both of which are important in conferring immunity to Toxoplasma gondii. The efficacy of genetic vaccination with a cDNA encoding the T. gondii SAG1 (P30) surface antigen was evaluated. Sera of immunized mice showed recognition of T. gondii tachyzoites by immunofluorescence and exhibited high titers of antibody to SAG1...

objective: escherichia coli o157:h7 is a gram-negative rod-shaped bacterium. e coli o157:h7 is an enterohemorrhagic (ehec) strain of the bacterium escherichia coli and a cause of foodborne illness. infection often leads to bloody diarrhea by producing a toxin called shiga toxin, which damages the intestines, and occasionally leads to kidney failure, especially in young children and elderly peop...

background and objectives: brucellosis is an important health problem in developing countries and no vaccine is available for the prevention of infection in humans. because of clinically infectious diseases and their economic consequences in human and animals, designing a proper vaccine against brucella is desirable. in this study, we evaluated the immune responses induced by a designed recombi...

Chlamydia trachomatis is one of the main causes of Sexually Transmitted Diseases (STDs) such as  prostatitis and epididymitis in men and cervicitis, endometriosis, vaginitis and ureogenital tract infections in women.  Serological tests with sensitivities related to specific antigens are commonly used as  routine laboratory tests for diagnosis of Chlamydia. In this research the Chlamydia Major O...