Granulosa cell tumors are representative of estrogenic ovarian tumors, and some Sertoli-stromal cell tumors are also estrogenic. The exact cells that are responsible for estrogenesis, however, have yet to be identified. In the present study, 25 sex cord-stromal tumors (20 granulosa cell tumors, 4 Sertoli-Leydig cell tumors, and a Sertoli cell tumor) were immunohistochemically examined for expre...

The cytodifferentiation and subcellular steroidogenic sites in the granulosa cell of the developing follicle and in vitro effect of estradiol-17 ~ (E2) on the granulosa cell of the preovulatory follicle in the human ovary were investigated using the electron microscopic cytochemistry. The follicular cell in the primordial follicle showed an elongated nucleus, rough endoplasmic reticulum, Golgi ...

The timing of cellular exit from the cell cycle during differentiation is specific for each cell type or lineage. Granulosa cells in the ovary establish quiescence within several hours after the ovulation-inducing luteinizing hormone surge, whereas they undergo differentiation into corpora lutea. The expression of Cdk inhibitors p21(Cip1/Waf1) and p27(Kip1) is up-regulated during this process, ...

Gonadotropin-releasing hormone (GnRH) has been found to be expressed within the ovary and to modulate cell differentiation in ovarian cells. In the present study we have analyzed the influence of GnRH on DNA synthesis in rat granulosa cells. Cells were obtained from immature DES-treated rats and cultured in defined medium (DMEM:F12) containing combinations of FSH, estradiol, and transforming gr...

The timing of cellular exit from the cell cycle during differentiation is specific for each cell type or lineage. Granulosa cells in the ovary establish quiescence within several hours after the ovulation-inducing luteinizing hormone surge, whereas they undergo differentiation into corpora lutea. The expression of Cdk inhibitors p21 and p27 is up-regulated during this process, suggesting that t...

Little is known about the pathogenesis of ovarian granulosa cell tumors. Recently, we reported the identification of a somatic FOXL2 402C-->G mutation that is present in virtually all adult-type granulosa cell tumors, but not in a wide range of other tumor types. This finding has important implications for the diagnosis and classification of ovarian sex cord-stromal tumors, provides insight int...

Sex-cord stromal tumours account for only about 6% of testicular neoplasms.' Leydig cell tumour is the commonest sub-type but other tissues that can be represented in varying amounts and degrees of differentiation include Sertoli, granulosa and theca-lutein elements. Testicular granulosa-cell tumour ofthe adult type is particularly uncommon. This report details a mixed sex-cord stromal tumour o...

objective(s): bone marrow-derived mesenchymal stem cells (bm-mscs) potentials make them appropriate for cell therapy including ability of differentiation and release of anti-inflammatory cytokines and growth factors secreta. for treatment of azoospermia to induce proliferation and differentiation of germ cells, mscs transplantation has been introduced. the aim of the present experimental case-c...

Ovarian inhibin production is stimulated by FSH and several TGFbeta family ligands including activins and bone morphogenetic proteins. Growth differentiation factor-9 (GDF-9) derived by the oocyte is a member of the TGFbeta/activin family, and we have previously shown that GDF-9 treatment stimulates ovarian inhibin-alpha content in explants of neonatal ovaries. However, little is known about GD...