ABSTRACT- Today, the authenticity of meat products with less costly and desirable species has increased. Therefore and considering religious, economicalor public health concerns, proper actions should be taken to prevent such frauds. In this study, real time PCR assay was applied for rapid, sensitive and specific identification and quantification of chicken tissue in meat products. Specific pri...

this study a novel High Performance Liquid Chromatography for the assay of nystatin in oral and vaginal tablets were optimized and validated using Box–Behnken experimental design. The method was performed in the isocratic mode on a RP-18 column (30 °C) using a mobile phase consisting of 0.05 M ammonium acetate buffer/ Methanol mixture (30:70) and a flow-rate of 1.0 ml/min. The method was valida...

Three rapid spectrophotometric methods were developed for the determination of sunitinib based on the formation of ion-pair complex in acidic medium with bromocresol purple, bromothymol blue, and bromophenol blue. The formed ion-pair complexes, extractable with chloroform, were measured at 422 nm for bromocresol purple, 425 nm for bromothymol blue and 427 nm for bromophenol blue. All these meth...

A flow injection method is reported for the determination of ascorbic acid based on amperometric detection, using a cobalt(II) phthalocyanine (CoPc) modified graphite-epoxy electrode. The amperometric respons was evaluated with regard to pH, ionic strength of the electrolyte, flow rate of the carrier solution, injected sample volume and conditioning time of the electrode. The limit of detec...

A rapid, sensitive and reproducible HPLC method using amperometric detector was developed and validated for the analysis of clarithromycin in human plasma. The separation was achieved on a monolithic silica column (MZ- C8 125×4.0 mm) using acetonitrile- methanol-potassium dihydrogen phosphate buffer (40:6:54,v/v), with pH of 7.5, as the mobile phase at a flow rate of 1.5 mL/min. The assay enabl...

Three rapid spectrophotometric methods were developed for the determination of sunitinib based on the formation of ion-pair complex in acidic medium with bromocresol purple, bromothymol blue, and bromophenol blue. The formed ion-pair complexes, extractable with chloroform, were measured at 422 nm for bromocresol purple, 425 nm for bromothymol blue and 427 nm for bromophenol blue. All these meth...

Determination of drug residues in food is of great importance due to their toxicity. Long-term exposure with low level of drug residues could be important, especially in children. Based on document study, oxytetracycline (OTC) is a prophylaxis and treatment agent for great number of diseases and possesses a broad spectrum activity against many pathogenic organisms and can be toxic or cause alle...

Easily degradating and various isomeric forms of rapamycin (Sirolimus) face the determination of this compound to many challenges. In this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic (RP-HPLC) method for rapamycin. Separation was performed on a C8 column (MZ, 15 × 4.6 mm, 5 µm particle size) using methanol:water (80:20 v/v) as the mobil...

In this manuscript, the electrocatalytic oxidation of doxepin (DOX) was studied at a carbon ionic liquid electrode, fabricated using graphite, and the ionic liquid 1-octylpyridinium hexaflourophosphate (OPFP). The surface of the proposed electrode was characterized by scanning electron microscopy. Differential pulse voltammetry was applied as an analytical technique for quantification of sub-mi...

Purpose: To develop and validate a spectrophotometric method for the quantitative determination of eflornithine hydrochloride as a pure compound and in pharmaceutical formulations. Methods: The method involved the reaction of the target compound with vanillin reagent at specific pH 5.6 to produce a green reddish color chromogen. The derivative chromogen exhibits absorption maxima at 578 nm. At ...