BACKGROUND The expression levels of bacterial genes can be measured directly using next-generation sequencing (NGS) methods, offering much greater sensitivity and accuracy than earlier, microarray-based methods. Most bioinformatics software for estimating levels of gene expression from NGS data has been designed for eukaryotic genomes, with algorithms focusing particularly on detection of splic...

Polyphenol oxidase and its isoenzymes are crucial enzymes in the tea tree that catalyze synthesis of theaflavins. In this study, polyphenol was used as research object, various protein sequence treatments, such TrxA fusion tag + N-terminal truncation, were tested for prokaryotic expression through Escherichia coli system. Comparative analyses conducted on activities different recombinant enzyme...

Bovine ephemeral fever is an acute and arthropod-borne viral disease of cattle and water buffalo which occurs seasonally in most of the world tropical and subtropical regions. The epizootic feature of the disease has been reported in Iran with serious economic consequences. The surface glycoprotein G of bovine ephemeral fever virus (BEFV) is composed of 4 antigenic sites (G1-G4) and plays the m...

The aim of this study was to investigate the role of the rat neuregulin-1 (NRG-1) protein in reducing doxorubicin (DOX)-induced myocardial toxicity and its underlying mechanism. The prokaryotic expression of the NRG-1 protein and the CCK8-determined activity of rat primary myocardial cells were evaluated under different DOX concentrations. Myocardial cells were divided into three groups: the co...

The super antibiotic bactericidal/permeability-increasing (BPI) protein is a member of a new generation of proteins that have been implicated as endotoxin-neutralizing agents. In this study, recombinant porcine BPI protein was obtained by generating porcine BPI encoding prokaryotic, eukaryotic, and yeast expression vectors. Recombinant protein expression was detected in yeast GS115, Escherichia...

The RUS (ROOT UVB SENSITIVE 1) proteins characterized by their unique DUF647 domain are widely distributed in eukaryotes. Their functional roles largely unknown except for the possible involvement of Arabidopsis RUS1 and RUS2 early seedling development. To investigate biochemical proteins, full length truncated were seamlessly fused with GFP cloned into prokaryotic expression vector pQE-100 whi...

Trichoderma reesei is a high-yield producer of cellulase for applications in lignocellulosic biomass conversion, but its production requires induction. A mixture glucose and β-disaccharide has been demonstrated to achieve high-level production. However, as inducers, β-disaccharides are prone be hydrolyzed by β-glucosidase (BGL) during fermentation, therefore need supplemented through feeding ov...