introduction: this study was designed to investigate the characters of the human endometrial epithelial cells when cultured on either plastic or ecm (extracellular matrix) gel surface and to study ultrastructural features of cultured cells in comparison to endometrial tissue. material and methods: human endometrial tissue was obtained from patients who had undergone hysterectomy for uterine myo...

introduction based on previous researches, dental pulp stem cells (dpscs) are easily accessible with limited morbidity after collection. their embryonic origin, from neural crests, explains their multipotency. dpscs are primarily derived from the pulp tissues of the teeth.objective: this study was undertaken to isolate, culture, and characterize two different third molar and first premolar huma...

Cell culture is a widely used technique in biomedical research. It permits the analysis of cell specific functions which can relate to changes in certain disease states at the single cell level. A number of cell culture models have been established. These include immortalised cell lines which replicate indefinitely in culture and retain the ability to differentiate and primary cell lines which ...

objective(s): sertoli cells support in vivo germ cell production; but, its exact mechanism has not been well understood. the present study was designed to analyze the effect of sertoli cells in differentiation of mouse embryonic stem cells (mescs) to germ cells.   materials and methods: a fusion construct composed of a stra8 gene promoter and the coding region of enhanced green fluorescence pro...

In cancer research, the use of established cell lines has gradually been replaced by primary cell cultures due to their better representation of in vivo cancer cell behaviors. However, a major challenge with primary culture involves the finding of growth conditions that minimize alterations in the biological state of the cells. To ensure reproducibility and translational potentials for research...

humans suffer transient or persistent starvation due to a lack of food intake, either because of fasting, voluntary dieting, or due to the scarcity of available food. at the cellular level it is possible to possess pathological starvation during ischemia and solid tumors. blood provides many nutrients to our cells, and researchers provide these nutrients to cells in culture in the form of enric...