نتایج جستجو برای: potato transgenic

تعداد نتایج: 81743  

Journal: :Journal of experimental botany 2006
Mohammad-Reza Hajirezaei Sophia Biemelt Martin Peisker Anna Lytovchenko Alisdair R Fernie Uwe Sonnewald

The aim of this work was to investigate the importance of cytosolic phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPC) in potato carbohydrate metabolism. For this purpose, the cytosolic isoform of phosphorylating GAPC was cloned and used for an antisense approach to generate transgenic potato plants that exhibited constitutively decreased GAPDH activity. Potato lines with decreased...

2014
Chang-Kug Kim Hye-Min Lim Jong-Kuk Na Ji-Weon Choi Seong-Han Sohn Soo-Chul Park Young-Hwan Kim Yong-Kab Kim Dool-Yi Kim

We introduced a multistep screening method to identify the genes in plants using microarrays and ribonucleic acid (RNA)-seq transcriptome data. Our method describes the process for identifying genes using the salt-tolerance response pathways of the potato (Solanum tuberosum) plant. Gene expression was analyzed using microarrays and RNA-seq experiments that examined three potato lines (high, int...

Journal: :Plant physiology 2006
Chihiro Yamamizo Kazuo Kuchimura Akira Kobayashi Shinpei Katou Kazuhito Kawakita Jonathan D G Jones Noriyuki Doke Hirofumi Yoshioka

Late blight, caused by the notorious pathogen Phytophthora infestans, is a devastating disease of potato (Solanum tuberosum) and tomato (Solanum lycopersicum), and during the 1840s caused the Irish potato famine and over one million fatalities. Currently, grown potato cultivars lack adequate blight tolerance. Earlier cultivars bred for resistance used disease resistance genes that confer immuni...

2017
Nurun Nahar Erik Westerberg Usman Arif Alexandre Huchelmann Alexandra Olarte Guasca Lisa Beste Kerstin Dalman Paresh C. Dutta Lisbeth Jonsson Folke Sitbon

Steroidal glycoalkaloids (SGA) are sterol-derived neurotoxic defence substances present in several members of the Solanaceae. In the potato (Solanum tuberosum), high SGA levels may render tubers harmful for consumption. Tuber SGA levels depend on genetic factors, and can increase as a response to certain stresses and environmental conditions. To identify genes underlying the cultivar variation ...

1997

Methods were developed to monitor persistence of genomic DNA in decaying plants in the field. As a model, we used recombinant neomycin phosphotransferase II (rNPT-II) marker genes present in genetically engineered plants. Polymerase chain reaction (PCR) primers were designed, complementary to 20-bp sequences of the nopaline synthase promoter in a transgenic tobacco and the cauliflower mosaic vi...

Journal: :Plant physiology and biochemistry : PPB 2011
Myoung Duck Kim Yun-Hee Kim Suk-Yoon Kwon Bo-Young Jang Sang Yeol Lee Dae-Jin Yun Ji-Hong Cho Sang-Soo Kwak Haeng-Soon Lee

Oxidative stress is one of the major causative factors for injury to plants exposed to environmental stresses. Plants have developed diverse defense mechanisms for scavenging oxidative stress-inducing molecules. The antioxidative enzyme 2-cysteine peroxiredoxin (2-Cys Prx) removes peroxides and protects the photosynthetic membrane from oxidative damage. In this study, transgenic potato (Solanum...

2013
Ravinder K. Goyal Robert E. W. Hancock Autar K. Mattoo Santosh Misra

Antimicrobial cationic peptides (AMPs) are ubiquitous small proteins used by living cells to defend against a wide spectrum of pathogens. Their amphipathic property helps their interaction with negatively charged cellular membrane of the pathogen causing cell lysis and death. AMPs also modulate signaling pathway(s) and cellular processes in animal models; however, little is known of cellular pr...

Journal: :Journal of experimental botany 2006
Matthew A Hannah Ellen Zuther Kerstin Buchel Arnd G Heyer

Raffinose family oligosaccharides (RFOs) are involved in the storage and transport of carbon and serve as compatible solutes for protection against abiotic stresses like drought or cold. RFOs are usually transported in plant species that load sugars symplastically into the phloem. Loading probably occurs by a polymer trapping mechanism which establishes a concentration gradient of assimilates b...

2012
Qingzhu LI Yansu LI Chaohan LI Xianchang YU

As an important antioxidant for plants and humans, L-ascorbic acid (AsA, vitamin C) can scavenge reactive oxygen species (ROS) and can be regenerated from its oxidized form in a reaction catalyzed by dehydroascorbate reductase (DHAR). To analyse the effect of overexpressing DHAR on tomato (Solanum lycopersicum), an expression vector containing potato cytosolic DHAR (DHAR1) or chloroplastic DHAR...

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