In this paper we describe how a robotic arm can acquire new skills, and specifically how to play beer pong. We used two different optimization algorithms are described and applied to teach a robot how to play the game beer pong. The first algorithm is the gradient based finite difference method. The second one is the expectation maximization algorithm PoWER. Their performance, consisting of bot...

http://dx.doi.org/10.1016/j.displa.2014.05.001 0141-9382/ 2014 Elsevier B.V. All rights reserved. ⇑ Corresponding author. Address: Institute of Electronic Engineering, National Yunlin University of Science & Technology, No. 123 Sec.3 University Rd. Douliou, Yunlin County 64002, Taiwan ROC. Tel.: +886 5 5342601x4344; fax: +886 5 5321792. E-mail addresses: [email protected] (C.-Y. Chen), g9...

Pyruvate, phosphate dikinase from Bacteroides symbiosus catalyzes the conversion of pyruvate, ATP, and Pi to Penolpyruvate, AMP, and PP,. Initial velocity studies by both the forward and reverse reactions in which the three substrates were varied in a constant ratio were found to be in accord with a Tri Uni Uni Ping Pong mechanism. The reaction involves three partial reactions which were invest...

Alkyldihydroxyacetone-P (alkyl-DHAP) synthase, the enzyme that forms the ether linkage of alkyl and alk-1-enyl glycerolipids, has been solubilized from ehrlich ascites cell microsomes using Triton X-100 and acetone precipitation. the solubilized enzyme, which is stabilized by glycerol or ethylene glycol, was then purified further by chromatography on DEAE-cellulose, QAE-Sephadex, Matrex Red, an...

The proteinase-catalysed synthesis of [Leu]enkephalin and [Met]enkephalin was studied kinetically. Na-t-Butoxycarbonyl-amino acids and peptides or their ethyl esters served as acyl donors, and amino acid phenylhydrazides were used as acyl acceptors. Initial-velocity measurements of oa-chymotrypsin-catalysed peptide synthesis gave rise to kinetic patterns that are compatible with a ping-pong mec...

Human dihydroorotate dehydrogenase (huDHODH) is essential for de novo biosynthesis of pyrimidines and the target of two immunosuppressive drugs, brequinar and the leflunomide metabolite A77-1726 (Chen et al., 1992; Davis et al., 1996). Using a T7 RNA polymerase expression system, we produced huDHODH as a fusion protein containing an amino-terminal decahistidine tag. Escherichia coli growth and ...