The relative number of copies of pumpkin DNA sequences which are complementary to high molecular weight ribosomal RNA (HMW rRNA), 5S and 4S RNA was found, by DNA-RNA hybridization experiments, to be approximately 1:1.2:2.5. At saturation, the levels of hybridization obtained with these RNAs were 2.4%, 0.029% and 0.044%, respectively. Based upon a haploid genomic DNA content of 4.9 x 10^' dalton...

Various studies have shown that water-stressed plants are more tolerant of ozone exposures than are unstressed plants. Two probable explanations for this tolerance are (a) stomatal closure which reduces ozone uptake and (b) biochemical or anatomical changes within the leaves. Phaseolus vulgaris cv Pinto bean plants were established and transferred to membrane systems which controlled the osmoti...

The hypothesis that crown gall tumorigenesis requires the formation of spheroplasts was tested by comparing the tumorinducing ability of populations of both permanent and revertable spheroplasts of Agrobacterium tumefaciens with that of popula tions of the typical rod form of the bacterium. Permanent spheroplasts produced by serial transfers in medium containing 0.005 MD-methionine did not prod...

Computer analysis was used for the detection of rhythmic components and the estimation of period length in leaf movement records. The results of this study indicated that spectral analysis can be profitably used to determine rhythmic components in leaf movements.In Pinto bean plants (Phaseolus vulgaris L.) grown for 28 days under continuous light of 750 ft-c and at a constant temperature of 28 ...

Hyphae, 2 to 8 days postinoculation (dpi), and haustoria, 5 dpi, were isolated from Uromyces appendiculatus infected bean leaves (Phaseolus vulgaris cv. Pinto 111) and a separate cDNA library prepared for each fungal preparation. Approximately 10,000 hyphae and 2,700 haustoria clones were sequenced from both the 5' and 3' ends. Assembly of all of the fungal sequences yielded 3,359 contigs and 9...

Phenolic acids have been identified in a variety of legumes including lima bean, broad bean, common bean, pea, jack bean, goa bean, adzuki bean, hyacinth bean, chicking vetch, garbanzo bean, dral, cow bean, rice bean, mung bean and soybean. The present study was carried out with the following aims: (1) to identify and quantify the individual phenolic acid and determine the total phenolic conten...

In 2008 (Pinto da Costa, Alonso, & Cardoso, 2008) we proposed the unimodal paradigm for ordinal data classification and also a new coefficient to measure the performance of ordinal data classifiers; that is, classifiers for the important and often neglected case where there is an order relation between the classes. Many real life settings exist which involve classifying examples (instances) int...

The statistics of generalized F tests are quotients of linear combinations of independent chi-squares. Given a parameter, θ, for which we have a quadratic unbiased estimator, θ̃, the test statistic, for the hypothesis of nullity of that parameter, is the quotient of the positive part by the negative part of such estimator. Using generalized polar coordinates it is possible to obtain selective ge...

The antifungal activity of proteinaceous compounds from different food matrices was investigated. In initial experiments, water-soluble extracts of wheat sourdoughs, cheeses, and vegetables were screened by agar diffusion assays with Penicillium roqueforti DPPMAF1 as the indicator fungus. Water-soluble extracts of sourdough fermented with Lactobacillus brevis AM7 and Phaseolus vulgaris cv. Pint...

BACKGROUND AND AIMS The Asian genus Vigna, to which four cultivated species (rice bean, azuki bean, mung bean and black gram) belong, is suitable for comparative genomics. The aims were to construct a genetic linkage map of rice bean, to identify the genomic regions associated with domestication in rice bean, and to compare these regions with those in azuki bean. METHODS A genetic linkage map...