The effects of culture medium and serum components on immunoglobulin (Ig) production by mouse splenocytes were examined. In this study, we showed that culture medium containing charcoal-dextran-treated fetal bovine serum (CDFBS) supported Ig production more efficiently than culture medium containing FBS. In addition, RPMI 1640 medium supported Ig production more efficiently than Dulbecco modifi...

Adipose-derived stem cells (ADSCs) are an attractive cell source for tissue engineering, and recently a modified aggregate culture of human ADSCs (hADSCs) was established based on preparation of three-dimensional (3D) cell aggregates in growth factor-enriched low serum medium using the hanging droplet method. Growth and differentiation factor 5 (GDF5) plays a critical role in chondrogenesis and...

1. Mouse resident peritoneal macrophages in culture modified human 125I-labelled low-density lipoprotein (LDL) to a form that other macrophages took up about 10 times as fast as unmodified LDL. The modified LDL was toxic to macrophages in the absence of serum. 2. There was a lag phase of about 4-6 h before the LDL was modified so that macrophages took it up faster. A similar time lag was observ...

OBJECTIVE Worldwide, diabetes mellitus (DM) is an ever-increasing metabolic disorder. A promising approach to the treatment of DM is the implantation of insulin producing cells (IPC) that have been derived from various stem cells. Culture conditions play a pivotal role in the quality and quantity of the differentiated cells. In this experimental study, we have applied various culture conditions...

Giardia lamblia cysts were isolated from fecal samples of 55 symptomatic and asymptomatic patients with giardiasis. The cysts were harvested and purified using the sucrose gradient method. IS The excystation of G. lamblia was obtained by treating them with HCI (0.01N)', then they were transferred on to modified TYI-S-33 medium. Out of 55 stool samples 17 cases were excysted with a rate of ...

The Dvoretzky–Kiefer–Wolfowitz (DKW) inequality says that if Fn is an empirical distribution function for variables i.i.d. with a distribution function F , and Kn is the Kolmogorov statistic √ n supx |(Fn − F )(x)|, then there is a finite constant C such that for any M > 0, Pr(Kn > M) ≤ C exp(−2M2). Massart proved that one can take C = 2 (DKWM inequality) which is sharp for F continuous. We con...

The combination of culture on modified New York City (MNYC) medium and identification of neisserial isolates by the Phadebact gonococcus test was evaluated in routine laboratory practice. The sensitivity of coagglutination was 96.7% (318/329 isolates) and that of immunofluorescence (IF) 97.9% (322/329 isolates); the specificity of both methods was 96.8% (120/124 isolates). Of the 329 gonococcal...

Background: In vitro maturation and fertilization are techniques to increase efficiency of mammals reproduction. However, in vitro culture conditions exert an imbalance between oxidants and antioxidants which may reduce live birth and pregnancy rates when compare to in vivo conditions. Materials and Methods: The present study was conducted as a part of the ovine IVF program organized by Univers...