.....................................................................................................................4 Introduction/Background............................................................................................... 5 Developmental Nature of Breast Cancer...........................................................................6 Sources of Metals.............................

Genotoxicity studies on toxic metals and their organic compounds are very important, especially so in the investigation of the effects of these compounds on the aquatic environments where they tend to accumulate. The use of endemic aquatic organisms as biological sentinels has proved useful to environmental monitoring. We assessed the mutagenic potential of tributyltin (TBT) and inorganic lead ...

Bacteria exhibit the ability to metabolize a wide array of contaminants and, as a result, reduce their toxicity to wildlife and humans. The general goal of this project was to design and evaluate, as a proof-of concept, a DNA microarray assay to evaluate bacterial detoxification mechanisms in contaminated sediments. The assay consists of 100 probes that measure activity of bacterial remediation...

Radiocesium accumulated in the soil by nuclear accidents is a major environmental concern. The transport process of cesium (Cs+) tightly linked to indispensable plant nutrient potassium (K+) as they both belong group I alkali metals with similar chemical properties. Most transporters that had been characterized date Cs+ are directly or indirectly K+. Using combinatorial approach physiology, gen...

Using the phoA-fusion technology, the recombinant metallothionein (MT) from freshwater crab (Sinopotamon yangtsekiense) has been successfully produced in Escherichia coli. MT purified from the bacterial suspension showed one polypeptide with a molecular weight of 7 kDa by tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Tricine-SDS-PAGE). Western-blotting confirmed the polypep...

The current study describes a method for assessing the oxidative potential of common environmental stressors (ambient air particulate matter), using a plasmid relaxation assay where the extract caused single-strand breaks, easily visualised through electrophoresis. This assay utilises a miniscule amount (11 µg) of particulate matter (PM) extract compared to other, cell‑based methods (~3,000 µg)...

Lipid peroxidation (LPO), an oxidative chain reaction often occurring in the human body and food, is harmful to health. Due its convenience low cost, this guideline highlights benefits drawbacks of LPO models by spectrophotometer vitro vivo. The preparation process mechanism lipid substrates, oxidation triggers detection methods are discussed paper better screen antioxidants. substrates mostly ...

background: holothuroids (sea cucumbers) are members of the phylum echinodermata, which produce saponins. saponins exhibit a wide spectrum of pharmacological and biological activities. in this study, we isolated the crude saponins from the body wall of the dominant iranian species of sea cucumber, holothuria leucospilota (h. leucospilota). the purpose of this study was to confirm the presence o...

background: alpinia galanga l. belonging to the family zingiberaceae is widely grown in the state of kerala, india. they are effective antioxidant and free radical scavenger under both in vitro and in vivo condition. the efficacy of the isolated flavonoids in conferring protection from radiation induced damages to genomic dna was studied. materials and methods: the bioflavonoids, galangin and k...