Background: The number of prokaryotic genome sequences becoming available is growing steadily and is growing faster than our ability to accurately annotate them. Description: We describe a fully automated service for annotating bacterial and archaeal genomes. The service identifies protein-encoding, rRNA and tRNA genes, assigns functions to the genes, predicts which subsystems are represented i...

The small subunit (SSU) ribosomal RNA (rRNA) is the most commonly used marker for identification of microbial taxa, but its full-length reconstruction from high-throughput sequencing (HTS) data remains challenging. Metagenomics and total (total RNA-Seq) are target-PCR-free HTS methods that to characterize communities simultaneously reconstruct SSU rRNA sequences. However, more testing required ...

We report a partial genome sequence for the Coxiella-like endosymbiont strain CLE-RmD, assembled from metagenomics data obtained from the southern cattle tick (Rhipicephalus microplus) Deutsch strain.

The current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak demonstrates the potential of coronaviruses, especially bat-derived beta coronaviruses to rapidly escalate a global pandemic that has caused deaths in order several millions already. huge efforts put place by scientific community address this emergency have disclosed how implementation new technologies is crucial ...

Successful screening of enzyme libraries in functional metagenomics and directed evolution becomes more likely after uniform cell growth droplets amplifies genotype phenotype.

How can we determine the distribution of uncultured marine microorganisms? Targeted metagenomics has provided the complete chloroplast genome sequence, and the distribution, for a picoplanktonic pelagophyte alga.

Obtaining an unbiased view of the phylogenetic composition and functional diversity within a microbial community is one central objective of metagenomic analysis. New technologies, such as 454 pyrosequencing, have dramatically reduced sequencing costs, to a level where metagenomic analysis may become a viable alternative to more-focused assessments of the phylogenetic (e.g., 16S rRNA genes) and...

The microbiome is a complex community of Bacteria, Archaea, Eukarya, and viruses that infect humans and live in our tissues. It contributes the majority of genetic information to our metagenome and, consequently, influences our resistance and susceptibility to diseases, especially common inflammatory diseases, such as type 1 diabetes, ulcerative colitis, and Crohn's disease. Here we discuss how...

Whether they are exposed to extremes of heat or cold, or buried deep beneath the Earth's surface, microorganisms have an uncanny ability to survive under these conditions. This ability to survive has fascinated scientists for nearly a century, but the recent development of metagenomics and 'omics' tools has allowed us to make huge leaps in understanding the remarkable complexity and versatility...