Lipofuscln content was determined in the brains of 41 American lobsters Homarus amencanus (Milne-Edwards) aged 4, 13 and 27 mo reared individually at 19 to 20°C. Llpofuscin was quantified by fluorescence microscopy and image analysis. Lipofuscin granules occurred In each age group and in the oldest group appeared as large aggregations collectively averaging 2.1 % of the area of histological sec...

AGED-RELATEDACCUMULATION OF LIPOFUSCIN IN THE BRAIN OF DOGS S. Nesic*, B. Risti c y and M. Jovanovi c* *Faculty of Veterinary Medicine, University of Belgrade, Belgrade and yVeterinary Specialized Institute Zaje car, Zaje car, Serbia Introduction: Lipofuscin is a pigment that accumulates in the process of ageing, mainly in post-mitotic long living cells (e.g. neurons and myocytes). The pigment ...

PURPOSE To determine whether drusen in patients with age-related maculopathy and macular degeneration (ARM/AMD) are associated with focal changes in retinal pigment epithelium (RPE) lipofuscin fluorescence. METHOD A new autofluorescence imaging device was used to study lipofuscin distribution associated with individual drusen in 20 patients with ARM/AMD. Paired monochromatic and autofluoresce...

Kufs' disease or NCL4 (neuronal ceroid lipofuscinosis type 4) is a rare and poorly characterized, adult-onset form of NCL. The mutation in gene CLN, underlying Kufs' disease, still remains unknown. The diagnosis of this disease is difficult because it is based only on clinical and ultrastructural examinations. We report the case of a 45-year-old woman referred to the Neurological Department wit...

Hypocholesterolemic activity of dietary polyunsaturated fatty acids is observed after relatively short-term but not long-term feedings, and their long-term feedings are suspected to accelerate aging through tissue accumulation of lipid peroxides and age pigments (lipofuscin). To define the long-term effects of fats and oils in more detail, female mice were fed a conventional basal diet suppleme...

Several lines of investigation suggest that the nondegradable fluorophores that accumulate as lipofuscin in retinal pigment epithelium (RPE) cells contribute to the etiology of macular degeneration. Despite evidence that much of this fluorescent material may originate as inadvertent products of the retinoid cycle, the enzymatic pathway by which the 11-cis-retinal chromophore of rhodopsin is gen...

An ultrastructural and quantitative study of the age-related changes occurring in the relay neurons of the dorsal lateral geniculate nucleus (dLGN) was carried out using male Wistar rats aged 3, 18, 24, and 28 months. Morphometric techniques were used to obtain data regarding cellular activity including soma, nuclear, and nucleolar size. Volume fractions for rough endoplasmic reticulum (RER), m...

Oxidative mechanisms are considered to contribute to the aging changes in retinal pigment epithelial (RPE) cells that underlie the pathogenesis of age-related macular degeneration. An important source of oxidative damage is likely to be the photoreactive pigments that progressively accumulate and constitute the lipofuscin of retinal pigment epithelial cells. Evidence for a link between RPE lipo...