نتایج جستجو برای: isolation mitochondria
تعداد نتایج: 176965 فیلتر نتایج به سال:
background: friedreich ataxia (frda) is an autosomal recessive disorder caused by guanine-adenine-adenine (gaa) triplet expansions in the fxn gene. its product, frataxin, which severely reduces in frda patients, leads to oxidative damage in mitochondria. the purpose of this study was to evaluate the triple nucleotide repeated expansions in iranian frda patients and to elucidate distinguishable ...
background: the mitochondria are an important source of adenosine triphosphate (atp) production in pre-implantation embryo. therefore, the objective of this study was to investigate the effect of vitrification and in vitro culture of mouse embryos on their mitochondrial distribution and atp content. methods: the embryos at 2-pn, 4-cell and blastocyst stages were collected from the oviduct of st...
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1. DNA prepared from non-gelable rat liver nuclei isolated in the presence of disrupted mitochondria at pH 6.0, has been compared with DNA obtained from gelable nuclei isolated at pH 4.0. The DNA of the non-gelable nuclei is partially depolymerized relative to the DNA of the gelable nuclei. 2. It has been found that sufficiently small quantities of crystallized DNAase I can cleave a very large ...
Studying organelles in isolation has been proven to be indispensable for deciphering the underlying mechanisms of molecular cell biology. However, observing organelles in intact cells with the use of microscopic techniques reveals a new set of different junctions and contact sites between them that contribute to the control and regulation of various cellular processes, such as calcium and lipid...
The majority of the dehydrogenases of mammalian mitochondria are linked to the respiratory chain (cytochrome system) by way of pyridine nucleotides. Although the intramitochondrial pyridine nucleotide appears to be tightly bound in intact mitochondrial preparations (1) and thus does not function as a mobile coenzyme, the requirement of these dehydrogenases for added diphosphopyridine nucleotide...
The majority of the dehydrogenases of mammalian mitochondria are linked to the respiratory chain (cytochrome system) by way of pyridine nucleotides. Although the intramitochondrial pyridine nucleotide appears to be tightly bound in intact mitochondrial preparations (1) and thus does not function as a mobile coenzyme, the requirement of these dehydrogenases for added diphosphopyridine nucleotide...
The exocrine pancreas, an active secretory gland, is a suitable model for studies concerning the structure of cell organelles and the coupling of energy production with different biosynthetic processes (1, 2) . However, the investigation of isolated pancreas mitochondria is not often performed because of the many difficulties in obtaining reproducible preparations with well-preserved structure ...
The isolation of a new class of mutants permitting facultative anaerobiosis in Neurospora crassa is described. Backcross analyses to the obligate aerobe prototroph (An(-)) indicate single nuclear gene inheritance (An(-)/An(+)). An(+) and An(-) are indistinguishable in morphology and growth rates under aerobic conditions. Anaerobic growth requires nutritional supplements that are dispensable for...
Obvious lack of interfibrillar mitochondria (IFM) sub population in isolated mitochondria attributed to limitations in isolation procedure. In this manuscript, we compared the functional activities of mitochondrial sub-populations namely, IFM and sub-sarcolemmal (SSM) mitochondria obtained from isolated rat heart subjected to different perfusion time namely (minutes) 0.5,5,10,25,60 and 120. Pro...
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