نتایج جستجو برای: intercalator

تعداد نتایج: 371  

Journal: :Nucleic acids research 2004
Junko Wakai Atsuko Takagi Masato Nakayama Takahito Miya Takatoshi Miyahara Tsuyoshi Iwanaga Shigeori Takenaka Yasuyuki Ikeda Masahiko Amano Tomohiro Urata

We describe the development of a new type of DNA array chip that utilizes electrochemical reactions and a novel method of simultaneously identifying multiple genetic mutations on an array chip. The electrochemical array (ECA) uses a threading intercalator specific to double-stranded nucleotides, ferrocenylnaphthalene diimide (FND), as the indicator. ECA does not require target labeling, and the...

2017
Soumi Biswas Satyabrata Samui Arpita Chakraborty Sagar Biswas Debapriya De Utpal Ghosh Apurba K. Das Jishu Naskar

The report describes the synthesis, self-association and DNA binding studies of an aromatic tripeptide H-Phe-Phe-Phe-OH (FFF). The peptide backbone adopts β-sheet conformation both in solid and solution. In aqueous solution, FFF self-assembles to form nanostructured aggregates. Interactions of this peptide with calf-thymus DNA (ct-DNA) have been studied using various biophysical techniques incl...

Journal: :Chemical biology & drug design 2008
John J Bowling Hari K Pennaka Kelly Ivey Subagus Wahyuono Michelle Kelly Raymond F Schinazi Frederick A Valeriote David E Graves Mark T Hamann

Aaptamine has potent cytotoxicity that may be explained by its ability to intercalate DNA. Aaptamine was evaluated for its ability to bind to DNA to validate DNA binding as the primary mechanism of cytotoxicity. Based on UV-vis absorbance titration data, the K(obs) for aaptamine was 4.0 (+/-0.2) x 10(3) which was essentially equivalent to the known DNA intercalator N-[2-(diethylamino)ethyl]-9-a...

Journal: :Nucleic acids research 1997
J N Lampe I V Kutyavin R Rhinehart M W Reed R B Meyer H B Gamper

G/A motif triplex-forming oligonucleotides (TFOs) complementary to a 21 base pair homopurine/homopyrimidine run were conjugated at one or both ends to chlorambucil. These TFOs were incubated with several synthetic duplexes containing the targeted homopurine run flanked by different sequences. The extent of mono and interstrand cross-linking was compared with the level of binding at equilibrium....

2017
Sara Bonetta Cristina Pignata Silvia Bonetta Lorenza Meucci Donatella Giacosa Elena Marino Giorgio Gilli Elisabetta Carraro

Legionella pneumophila is a ubiquitous microorganism widely distributed in aquatic environments and can cause Legionellosis in humans. A promising approach to detect viable cells in water samples involves the use of quantitative polymerase chain reaction (qPCR) in combination with photoactivatable DNA intercalator propidium monoazide (PMA). However, the PMA efficiency could be different dependi...

Journal: :The journal of physical chemistry. B 2007
Debapriya Banerjee Samir Kumar Pal

In the present study, steady-state, picosecond time-resolved fluorescence and polarization gated anisotropy have been used to establish simultaneous binding of an intercalator (ethidium bromide, EtBr) and a minor groove binder (Hoeschst 33258, H258) to a dodecamer DNA of specific sequence. The Förster resonance energy transfer (FRET) studies between the dyes H258 (donor) and EtBr (acceptor) in ...

Journal: :Chemosensors 2023

Forced intercalation (FIT) probes have proven to be a reliable, rapid, inexpensive, and accurate method for the detection visualization of specific nucleic acid sequences. The accommodation rationally designed chromone-based fluorogen within double-stranded DNA structure was investigated by UV–Vis spectrophotometry steady-state fluorescence spectroscopy under physiological pH conditions. After ...

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