background:the diagnostic accuracy of frozen section as an important source of information in surgical pathology is important not only in the management of surgical patients but also as a measure of quality control in surgical pathology. in this study, we evaluated the diagnostic accuracy of frozen sections over a 6-year period in a teaching hospital in iran. methods: we retrospectively review...

introduction: acanthamoeba is an opportunistic protist, which is ubiquitously distributed in the environment. infection with acanthamoeba spp. poses threat to human health, such as, acanthamoeba keratitis (ak) that is a vision-threatening infection of the cornea. this study aimed to identify the species of acanthamoeba strains isolated from cornea of keratitis patients by polymerase chain react...

PURPOSE To investigate increased free radical activity and the accumulation and localization of lipid peroxidation in the anterior segment of the eye with uveitis. METHODS Experimental autoimmune uveitis (EAU) was induced in rats using human S-antigen peptide. Conjugated dienes (CD) and keto-dienes (KD) were then extracted from the cornea, iris-ciliary body and lens of the EAU eyes. The quant...

during spring, summer and winter seasons, sistani donor cows, with normal reproductive status, were superovulated and embryos were recovered non-surgically on day 7. grade a blastocyst embryos were either transferred fresh (spring) or frozen (summer and winter). recovered embryos during summer and winter were exposed to glycerol and frozen using conventional method. during spring season, recipi...

Purpose: During the latter stages of development, the secondary chick corneal stroma undergoes significant structural and compositional changes. The current study was designed to provide information about the orientation of collagen molecules (and thus fibrils) as the cornea matures before hatching. Collagen orientation is an important biomechanical parameter given that collagen lamallae consis...

PURPOSE To compare cryopreserved human corneal endothelial cells (HCECs) grown in human serum-supplemented media (HS-SM) with cryopreserved HCECs grown in fetal bovine serum-supplemented media (FBS-SM). METHODS Three pairs of human corneas from donors aged 8, 28, and 31 years were obtained from the eye bank. From each pair, one cornea was used to start a HCEC culture using HS-SM; the other co...

OBJECTIVE To examine the prevalence of and risk factors for cornea guttata in a rural southwestern island of Japan. DESIGN Cross-sectional, population-based study. All residents of Kumejima Island, Japan, located in southwestern Japan (eastern longitude, 126° 48'; northern latitude, 26° 20'), 40 years or older were asked to undergo a comprehensive questionnaire and ocular examination, includi...

Hydroxyproline (Hyp) concentrations (total, free, peptide-bound and protein-bound) in camel eye tissues were determined. Total Hyp concentration was highest in iris, followed by ciliary body, sclera, cornea, lens and retina; the difference between total Hyp concentration of iris and sclera (P < 0.05) and cornea, lens and retina (P < 0.001) was statistically significant. Cornea had the highest c...

PURPOSE To compare the abundance and spatial distribution of serum albumin in the mouse and bovine cornea. METHODS Serum albumin from cornea was separated from transketolase by SDS-PAGE (+/-dithiothreitol [DTT]) and identified by peptide sequencing and immunoblot analyses. The fractional content of serum albumin was determined in water-soluble extracts of cornea by imaging analyses after SDS-...

PURPOSE To isolate tissue-specific gene products that contribute to corneal integrity. METHODS A cDNA library was constructed and differentially hybridized. Cornea-specific clones were purified and further characterized. RESULTS In this study cornea-specific gene products were isolated by differential cDNA hybridization. In addition to known cornea-specific gene products, a transcript was i...