The contractile dysfunction of the hibernating myocardium in situ results from local environmental factors, but also from intrinsic cellular remodelling that may determine reversibility. Previous studies have suggested defects in myofilament Ca2+ responsiveness. We prepared single myocytes from control (CTRL, n(pigs)=7) and from hibernating myocardium (HIB, n(pigs)=8), removed the membranes and...

Replication protein A (RPA) is a ubiquitous eukaryotic single-stranded DNA (ssDNA) binding protein that serves to protect ssDNA from degradation and annealing, and as a template for recruitment of many downstream factors in virtually all DNA transactions in cell. During many of these transactions, DNA is tethered and is likely subject to force. Previous studies of RPA's binding behavior on ssDN...

Analysis of direct teleoperation with force feedback indicates that humans have difficulties perceiving the direction and amount of reflected force. Locally implemented force-based reactive behaviors provide accurate and timely feedback to assist in replicating human skills and dexterity. The contact forces between the slave arm tool and the environment is analyzed using three settings. First, ...

The single coiled torsion spring made of Ti-Ni shape memory alloy (SMA) is expected to be used as a that smaller in size and has larger displacement than springs conventional materials. However, heat-treatment necessary for SMA memorize the shape. Furthermore, it well known mechanical properties are varied with variation condition. Therefore, characteristics affected by In this study, effects c...

Cardiac tissue engineering strategies are based on the development of functional models of heart muscle in vitro. Our research is focused on evaluating the feasibility of different tissue engineering platforms to support the formation of heart muscle. Our previous work was focused on developing three-dimensional (3D) models of heart muscle using self-organization strategies and biodegradable hy...

The objective of this study was to determine the effect of sampling methodology on the relationship between longissimus tenderness and measures of biochemical meat traits. Sampling methodology included measurements of sarcomere length, collagen concentration, and postmortem desmin proteolysis on raw samples and measurements of these same traits on the same cooked meat used for shear force measu...