We used a red chromophore formation pathway, in which the anionic red chromophore is formed from the neutral blue intermediate, to suggest a rational design strategy to develop blue fluorescent proteins with a tyrosine-based chromophore. The strategy was applied to red fluorescent proteins of the different genetic backgrounds, such as TagRFP, mCherry, HcRed1, M355NA, and mKeima, which all were ...

The use of biodegradation methods by microorganisms in the removal of industrial dyes are widely considered owing to their high efficiency and compatibility to the environment. Therefore, this study aims to evaluate the biodegradation of methylene blue (MB) from aqueous solution by bacteria isolated from contaminated soil. This study was performed in laboratory scale on bacteria isolated and pu...

Objectives Pyocyanin is a blue-greenish redox-active pigment, produced by Pseudomonas aeruginosa, with a wide range of biological and biotechnological applications. Pyocyanin biosynthesis is regulated by the quorum-sensing (QS) system in which the expression of QS genes and QS-controlled virulence genes may be affected by serum as a complex medium. In the current study, effects of adult bovine ...

OBJECTIVE The aim of this study was to assess the in vitro cytotoxicity of acrylic resins of different colors over time. METHODS Specimens were divided into 4 groups (n = 6) according to the color of the acrylic resin (Orto Class, Clássico, Campinas, São Paulo, Brazil): Group 1, clear acrylic resin; Group 2, pink acrylic resin; Group 3, blue acrylic resin; and Group 4, green acrylic resin. Al...

OBJECTIVES To evaluate existing protocols, based on Alamar blue (resazurin), for the routine determination of drug susceptibility in trichomonads, develop new ones and validate these by screening small antiprotozoal libraries. METHODS The resazurin-based assay was evaluated by determining fluorescence development in Trichomonas media with various cell densities after various intervals and in ...

We describe a blue/green inorganic material, Ba3(P1−xMnxO4)2 (I) based on tetrahedral MnO3− 4 :3d 2 chromophore. The solid solutions (I) which are sky-blue and turquoise-blue for x ≤ 0·25 and dark green for x ≥ 0·50, are readily synthesized in air from commonly available starting materials, stabilizing the MnO3− 4 chromophore in an isostructural phosphate host. We suggest that the covalency/ion...

this study evaluates the effects of spirulina (arthrosphia maxima) and carrots (daucus carota) powder on body color changes of 330 pseudoteropheus zebra with average 15 g initial weight, in 35 days experimental period. as the results showed, the best content of steamed carrot in wet diet was 20 %, and all the factors except background color showed significant differences with control treatment ...

In this work, scanning electron microscopy (SEM), X-ray diffraction (XRD), and infrared spectroscopy (FTIR) techniques were used to study blue, red, ochre pigments from wall paintings of the 16th century colonial convent San Nicolás de Tolentino in Actopan, Hidalgo, Mexico. blue pigments, nanometric fibers with a chemical composition mostly O, Si, Al, Mg identified. XRD FTIR analysis indicated ...

The indole tolerance level of Pseudomonas sp. strain ST-200 was 0.25 mg/ml. The level was raised to 4 mg/ml when diphenylmethane was added to the medium to 20% by volume. ST-200 grown in this two-phase culture system containing indole (1 mg/ml) and diphenylmethane (0.2 ml/ml) produced a water-soluble yellow pigment, isatic acid, and two water-insoluble and diphenylmethane-soluble pigments, blue...

Background This novel approach describes a rapid and simple method for identification of necrotic vs. viable cells within a mammalian blastocyst. MaterialsAndMethods Hatched bovine blastocysts produced in vitro were first incubated for 30 min in pre-equilibrated culture medium containing propidium iodide (PI; 300μg/ml) and bisbenzimide (Hoechst: H33342; 5μg/ml) fluorescent dyes. Embryos were th...