PRL-releasing peptide inhibits food intake after intracerebroventricular injection. PRL-releasing peptide immunoreactivity is found in several hypothalamic nuclei involved in feeding, with highest levels in the paraventricular and dorsomedial hypothalamic nuclei. The aim of this study was to examine the effect of PRL-releasing peptide on food intake after administration into these nuclei. Parav...

Human urinary disorders are generally studied in rodent models due to limitations of functional in vitro culture models of primary human urothelial cells (HUCs). Current HUC culture models are often derived from immortalized cancer cell lines, which likely have functional characteristics differ from healthy human urothelium. Here, we described a simple explant culture technique to generate HUCs...

The relationship between abscission and the evolution of ethylene and CO(2) was examined in explants and explant segments of cotton seedlings (Gossypium hirsutum L. cv. Acala SJ-1) under both static and flow system conditions, and in the presence and absence of mercuric perchlorate. Explant excision was immediately followed by increased ethylene evolution (wound ethylene); senescence was also a...

Hypericum perforatum L. (St. Johns’ wort) is the most commercially important species of the genus Hypericum and contains a wide range of components including naphthodianthrones, phloroglucinols, tannins, xanthones, phenolic acids and essential oil. In order to establish an efficient protocol for regeneration, the effects of explant type and plant growth regulators on direct and indirect shoot r...

Leaf explants of kaladium dwubarwne Caladium bicolor (Aiton) Vent., were cultured in vitro on MS media supplemented with 25 different concentrations of BAP and NAA in order to determine the appropriate concentrations for micropropagation. All combinations induced callus formation on explants. Callus production on leaves explants grown on control medium was very low. The medium enriched with 4 m...

evaluation of autogenous fibrinogen extracted from horse blood in fibrin glue and it's effects on horse's fibroblast and keratinocyte cells were preformed. fibrinogen has been obtained by glycine precipitation technique from horse's blood. fibroblast cells were separated by explant culture method from neck skin and keratinocytes were separated by enzyme digestion from lib samples...

An efficient In vitro protocol was established for callogenesis from various plant parts (Leaf, stem, flower bud and tendril) of Passiflora vitifolia Kunth. On Murashige Skoog (MS) basal medium supplemented with concentration auxins (NAA, IAA, 2,4-D) cytokinins (BAP Kn ). Different concentrations (0.5 to 4 mg/L), IAA 2mg/L), NAA 2 mg/l) cytokinin BAP mg/L) were assessed callus induction. Growth...

There are several differentiation methods for mesenchymal stem cells (MSCs) into hepatocyte-like cell. Investigators reported various hepatic differentiation protocols such as modifying culturing conditions or using various growth factors/cytokines. In this literature review, we compared different MSCs extraction and isolation protocols from Wharton’s jelly (WJ) and explored various MSCs differ...