Fluorogenic assays for a set of five hydrolytic enzymes involved in digestion and food utilization (alanine and arginine aminopeptidase, lipase/esterase, chitobiase, and beta-glucosidase) were optimized to measure activities of these enzymes in the same extracts of individual small North Sea copepods. The enzyme activities of Acartia clausi, Centropages typicus, Corycaeus anglicus, Paracalanus ...

(Cell Reports 33, 108294-1–9.e1–e4; October 20, 2020) In the originally published version of this article, supplemental information file containing Figures S1–S7 and Table S1 was inadvertently removed. The complete is now included with paper online. production team regrets error. Structural Functional Insights into an Archaeal Lipid SynthaseRen et al.Cell ReportsOctober 2020In BriefRen al. repo...

The cell wall of Mycobacterium tuberculosis (Mtb) consists of peptidoglycan, arabinogalactan and mycolic acids. The cytoplasmic steps in the peptidoglycan biosynthetic pathway, catalyzed by the Mur (A-F) enzymes, involve the synthesis of UDP-n-acetylmuramyl pentapeptide, a key precursor molecule required for the formation of the peptidoglycan monomeric building blocks. Mur enzymes are indispens...

Phospholipase A2 (PLA2) constitutes a diverse superfamily of enzymes which catalyze the deacylation of phospholipids. At least four types of PLA2 are potentially involved in arachidonic acid release in cells and tissues. Since all of them catalyze the same enzymatic reaction, it is difficult to distinguish between them in mixtures of enzymes normally present in biological samples. Utilizing spe...

The traditional Chinese medicines (TCMs) are essential components of alternative medicines. Many TCMs are known to alter the expression of hepatic drug-metabolizing enzymes and transporters. The molecular mechanism by which TCMs and/or their constituents regulate enzyme and transporter expression, however, has remained largely unknown. In this report, we show that two TCMs, Wu Wei Zi (Schisandr...

activities of thirty bacterial strains obtained from plants rhizosphere in different areas against phytophthora drechsleri were assessed through in vitro and greenhouse experiments. dual culture and extracellular metabolites tests were used in in vitro assays. the bacterial strains inhibited growth of p. drechsleri, the highest inhibition zones was belonged to t17-4 strain with 61.904% in dual ...

Abstract This work addresses the evaluation in vitro of different marine ( Dunaliella salina REC-0214B and Microchloropsis gaditana REC-0251B) freshwater Anabaena sp. BEA-0300B, Arthrospira platensis BEA-0007B, Chlorella vulgaris BEA-0753B, Spirogyra BEA-0666B) microalgae cyanobacteria as potential dietary ingredients aquafeeds. For this purpose, total protein content, amino acid composition, p...

The induction of enzymes is a defensive mechanism for some xenobiotics, but it may alter the drug's safety and efficacy by altering activity metabolic enzymes. One major families involved in phase I metabolism Cytochrome P450 (CYP) which get induced certain drugs. Concomitant administration drugs due to chronic disease or polypharmacy, inducers among them cause toxicity reduce plasma concentrat...

Protein ubiquitination has been historically associated with protein degradation, but recent studies have demonstrated other cellular functions substrate ubiquitination. Among the RING-type ubiquitin E3 ligase enzymes present in human genome, RNF167 is a transmembrane located endosomes and lysosomes implicated controlling endolysosomal pathway. Substrates of identified, ubiquitin-conjugating E2...

The performance of protease assays has been revolutionized by the advent of Xuorescence resonance energy transfer (FRET)-based assays. Generation of pure, reliable, high-quality substrate is essential to such assays. Small FRET substrates can be easily prepared by chemical production. The preparation of Xuorogenic substrate becomes cumbersome when a larger peptide substrate is required or when ...