BACKGROUND AND THE PURPOSE OF THE STUDY The quality of some of the human plasma derived drugs such as coagulation factor VIII and coagulation factor IX which can be used for the treatment of hemophilia A and B, depends on their activity which may be affected by filtration. In this study the quality of plasma with respect to coagulation factors FVII, FVIII, FIX, FV, FXI, Fibrinogen, antithrombin...

Serological methods serve as a direct or indirect means of pathogen infection diagnosis in plant and animal species, including humans. Dot-ELISA (DE) is an inexpensive sensitive, solid-state version the microplate enzyme-linked immunosorbent assay, with broad range applications epidemiology. Yet, its applicability limited by uncertainties qualitative output assay due to overlapping dot colorati...

A key obstacle in uncovering the orchestration between molecular and cellular events is the vastly different length scales on which they occur. We describe here a methodology for ultrastructurally mapping regions of cells and tissue as large as 1 mm(2) at nanometer resolution. Our approach employs standard transmission electron microscopy, rapid automated data collection, and stitching to creat...

An adhesion-promoting protein involved in the binding of Lactobacillus fermentum strain 104R to small intestinal mucus from piglets and to partially purified gastric mucin was isolated and characterized. Spent culture supernatant fluid and bacterial cell wall extracts were fractionated by ammonium sulfate precipitation and gel filtration. The active fraction was purified by affinity chromatogra...

Glucokinase is a monomeric protein under native and denaturating conditions yet presents a sigmoidal saturation function for glucose. These peculiarities suggested the possibility that polymerization occurs under assay conditions. Thus the apparent molecular weight of glucokinase was determined by gel filtration at 4°C and at 30°C in the presence of substrates and products, singly and in combin...