Pressure inactivation behavior of Bacillus amyloliquefaciens spores was investigated in deionized water. The spores of B. amyloliquefaciens were subjected to 105 degrees C and 700 MPa. The magnitude of the decrease in viability after pressure treatment was similar to that after pressure treatment followed by heat shock. The increase of dipicolinic acid (DPA) release was correlated with the spor...

The composition and application of a single, chemically defined medium or growth and sporulation of Bacillus subtilis is described. At 37 degrees C cells grew with a doubling time of about 40 min; cultures attained near-maximal spore formation (70 to 80% by 12 h after the end of exponential growth and produced 1 X 10(9) to 2 X 10(9) heat-resistant free spores at 24 h. Dipicolinic acid productio...

Dipicolinic acid (DPA) and the Ca2+ complex of DPA (CaDPA) are major chemical components of bacterial spores. With fluorescence being considered for the detection and identification of spores, it is important to understand the optical properties of the major components of the spores. We report in some detail on the room-temperature fluorescence excitation and emission spectra of DPA and its cal...

Actinide +VI complexes ( = , and ) with dipicolinic acid derivatives were synthesized characterized by powder XRD, SQUID magnetometry NMR spectroscopy. In addition, described first principles CAS based two-component spin-restricted DFT methods. The analysis of the 1H paramagnetic chemical shifts for all protons ligands according to X-rays structures shows that Fermi contact contribution is negl...

The initiation of germination of Bacillus megaterium QM B1551 spores, grown in supplemented nutrient broth, has been studied. The initiation properties depend on buffer concentrations and the particular batch of spores. Initiation in l-alanine, KBr, calcium dipicolinate, or in buffer alone increases as a function of the spore age; whereas initiation in glucose, l-leucine, or l-proline remains r...