A sulfated poly β-cyclodextrin (SPCD) modified silica-based monolithic column was prepared for enantiomeric separation. First, 2-hydroxy-3-allyloxy-propyl-β-cyclodextrin (allyl-β-CD) was bonded onto a bifunctional reagent 3-(methacryloxy)propyltriethoxysilane (γ-MAPS) modified silica-based monolith through radical polymerization; the column was then sulfated with chlorosulfonic acid. The SPCD c...

High-salt samples present a challenge to mass spectrometry (MS) analysis, particularly when electrospray ionization (ESI) is used, requiring extensive sample preparation steps such as desalting, extraction, and purification. In this study, infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) coupled to a Q Exactive Plus mass spectrometer was used to directly analyze 50...

The numbers of antiplasmin, antitrypsin and antichymotrypsin in human plasma and mutual relationship among them are discussed in this paper. Human blood plasma was divided into 2 major fractions by adding ammo nium sulfate, after desalting by sephadex, the fractions being further subfrac tionated by using column chromatographic procedure on DEAE-cellulose. The determination of antiproteolytic a...

We report a novel strategy to achieve simultaneous one-step in-situ self-desalting and enrichment (OISE) of peptides/proteins on a facilely fabricated patterned MALDI steel plate with a circular paraffin-steel-polystyrene structure. The OISE plate could efficiently segregate salts from both analytes and matrices while retaining both analyte and matrix concentrate, and facilitating them to form ...

A new 3D model of 1:1 salt ion transfer in the desalting channel an electrodialysis apparatus is presented and investigated this paper. For first time a three-dimensional mathematical taking into account electroconvection based on system Nernst – Planck, Poisson Navier Stokes equations with electric force natural boundary conditions proposed. To solve value problem, finite element method used c...

H����� V., M������ K., R������� M. (2004): Modern method of lactic acid recovery from fermentation broth. Czech J. Food Sci., 22: 87–94. Electrodialysis was used for lactic acid recovery from fermentation broth. In the first step, lactate was recovered and concentrated by desalting electrodialysis, and the second step was electroconversion of lactate to lactic acid by water-splitting electrodia...

Capabilities of mass spectrometry for the analysis of intact proteins can be increased through separation methods. Flow field-flow fractionation (FlFFF) is characterized by the particularly "soft" separation mechanism, which is ideally suited to maintain the native structure of intact proteins. This work describes the original on-line coupling between hollow-fiber FlFFF (HF FlFFF), the microcol...