An efficient protocol for in vitro shoot multiplication of Randia dumetorum (Emetic nut) has been developed. The seeds of R. dumetorum were germinated in vitro in MS medium in 5 weeks. Subsequent propagation using shoot tip as an explant was carried out in MS medium along with different concentrations and combinations of BAP (0.5-2.0) and NAA (0.0-2.0). Maximum shoot multiplication was obtained...

Calotropis procera, a plant of family Asclepiadaceae is of immense importance as multipurpose plant with medicinal as well as traditional uses. Latex of this plant has been used to cure disease like leprosy, syphilis, inflammation, cutaneous infections, and malarial fever and in many more. In vitro culture of this pharmacologically plant is required otherwise it leads the segregated loss of des...

Technique for rapid mass propagation of Geodorum densiflorum (Lam)Schltr. has been developed by using thin sections of stems of in vitro regenerated plantlets as explant source. Thin sections of stems (1.0-1.5 mm) when cultured in modified liquid and semisolid Knudson C (KnC) medium produced 1.8 and 1.2 protocorm like bodies (PLBs) per explant respectively. Peptone (2 g l-1) was effective in pr...

Crataeva religiosa G. Forst. var. nurvala (Buch.-Ham.) Hook. F. & Thomson (syn. Crataeva nurvala Buch.-Ham.), a fast growing medicinal tree species, has been successfully micropropagated under in vitro conditions. Direct multiple shoots were regenerated from nodal explants on Murashige and Skoog’s (MS) medium, solidified by agar and supplemented with 1 mg/L 6-benzyl amino purine (BAP) and 0.5 m...

Berberis tinctoria Lesch. Is an endemic plant to high hills of Nilgiris having lot of medicinal properties. For its better conservation through mass multiplication, attempts have been made to standardize tissue culture technology. The results of the study exhibited that the basal medium containing BAP and NAA each at 0.5 mg/1 was found to be the optimum for callus formation. Shoot proliferation...

In the present study examined the effects of different types of cytokinins on the growth of in vitro cultured carnation. NAA alone produced neither multiplication nor elongation. Combined with BAP, NAA increased multiplication. The biological activity of 4-PU-30 and especially that of thidiazuron was considerably higher than BAP’s activity. In equal concentrations with BAP thidiazuron increased...

The present studies report in vitro propagation of a rare and medicinally important orchid Vanda testacea through foliar explants. Explants were cultured on Mitra et al. (M) medium and its combinations with cytokinins (BAP, Kn 1 mgl each) and auxin (NAA 1 mgl) individually and in combination for initiation of regeneration response, proliferation of regenerants and subsequent development of plan...

Kenaf (Hibiscus cannabinus L.) is one of the important species of Hibiscus cultivated for fiber. Availability of homozygous parent lines is prerequisite to the use of the heterosis effect reproducible in hybrid breeding. The production of haploid plants by anther culture followed by chromosome doubling can be achieved in short period compared with inbred lines by conventional method that requir...

Elite genotypes from high yielding variety of Artemisia annua L. (Jeevanraksha, CIMAP Lucknow) were regenerated form nodal explants and maintained in MS culture medium with BAP and NAA. The leaves of tissue culture grown plants were inoculated with Agrobacterium rhizogenes strain LBA9402 containing the selective marker gene NPT II. Hairy roots emerged from the leaves, 8-10 days after inoculatio...