نتایج جستجو برای: culture supernatant

تعداد نتایج: 284354  

ژورنال: Medical Laboratory Journal 2017
Maftuni , Kazem , Zare , Peyman ,

ABSTRACT            Background and objective: Considering the toxic side effects of chemotherapy in treatment of cancer, anticancer drugs of natural origin including probiotic Lactobacillus strains have recently attracted a lot of attention. Methods: After culturing chronic myeloid leukemia cell line K562 in 96-well plates, effects of different concentrations of ...

Journal: :Asian Journal of Fisheries and Aquatic Research 2022

An experiment was conducted to evaluate the proximate composition of Spirulina platensis and growth performance in supernatant different concentrations (supernatant 20, 40 60 rotten guavas) digested guava medium (DRGM). The rates terms optical density, dry cell weight chlorophyll a spirulina were varied from each other. attained maximum 0.818 ± 0.003 mg/L 60% DRGM followed by 0.815 0.0015 0.809...

Journal: :Chinese medical journal 2015
Xin-Zhu Huang Pan-Pan Liang Han Ma Jin-Ling Yi Song-Chao Yin Zhi-Rui Chen Mei-Rong Li Wei Lai Jian Chen

BACKGROUND Trichophyton rubrum is superficial fungi characteristically confined to dead keratinized tissues. These observations suggest that the soluble components released by the fungus could influence the host immune response in a cell in contact-free manner. Therefore, this research aimed to analyze whether the culture supernatant derived from T. rubrum grown in the nail medium could elicit ...

Journal: :The Journal of Experimental Medicine 1983
J Fujimoto S Levy R Levy

Sensitive enzyme-linked immunosorbent assays (ELISA) for the detection of human T cell antigens in soluble form have been developed. The assays use mouse monoclonal antibodies and specific anti-Leu sera prepared in rabbits by immunizing with Leu antigens absorbed to monoclonal antibody affinity columns. With these assays, Leu-1, -2, and -3 antigen signals from extracts of as few as 5 X 10(3) ce...

Journal: :Journal of animal science 1997
S Saginala T G Nagaraja K F Lechtenberg M M Chengappa K E Kemp P M Hine

The efficacy and the optimum dose of Fusobacterium necrophorum crude leukotoxoid vaccine required to immunize and protect steers against experimentally induced liver abscesses were evaluated. The vaccine consisted of cell-free culture supernatant of a high leukotoxin-producing strain of F. necrophorum, inactivated with formalin and homogenized with an adjuvant. Twenty-five steers were assigned ...

Abbas Ghaderi, Mahboobeh Razmkhah Mansooreh Jaberipour Mojtaba Habibagahi Neda Mousavi Niri

Background: Several studies have demonstrated the immunosuppresive effects of mes-enchymal stem cells (MSCs) in allogeneic or mitogenic interactions. Cell-cell contact inhibition and secretion of suppressive soluble factors have been suggested in this re-gard. Objective: To investigate if adipose derived MSCs could inhibit Jurkat lym-phoblastic leukemia T cell proliferation during coculture. Me...

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