A gene encoding an S.pombe homologue of glycerol-3-phosphate dehydrogenase was isolated from a XEMBL3 library. The clone was originally isolated because it hybridised to a rat BiP cDNA probe (1). Upon sequencing, however, it was found that there was no homology between this clone and BiP at the amino acid level. The open reading frame was used to search databases and the gene identified as an S...

CD9 is a tetra-membrane-spanning glycoprotein involved in cell adhesion, migration, growth signaling and tumor cell metastasis. In this study a CD9 cDNA clone has been isolated from chick cDNA library. The chick CD9 clone contains an open reading frame of 224 amino acids. The deduced amino acid sequence from chick CD9 shows 63.3% identity with the human counterpart. Human and monkey CD9 upregul...

A cDNA clone encoding a cellular protein that interacts with murine leukemia virus (MuLV) Gag proteins was isolated from a T-cell lymphoma library. The sequence of the clone is identical to the C terminus of a cellular protein, KIF4, a microtubule-associated motor protein that belongs to the kinesin superfamily. KIF4-MuLV Gag associations have been detected in vitro and in vivo in mammalian cel...

Approximately 2.0 x 10 cDNA clones of an Schistosoma mansoni lambda gt11 cDNA library were screened in duplicate with serum from infected mice corresponding to distinct phases of infection. A cDNA clone (7/1) was isolated and recognized only by seven week serum. The clone was subcloned in pGEX-2T and Western-blot studies showed a specific antigenic expression confirming that only serum from the...

We have constructed a cDNA clone library from poly(A)+ RNA of very-late-period choriogenic silkmoth follicles. Clone DNAs that hybridize preferentially to RNA from the aeropyle crown region of the follicle (versus the flat region) were selected, and all could be placed in one of two homology groups. The two groups represent sequences encoding the very-late-period chorion proteins E1 and E2; thi...

We have isolated several cDNA's complementary to gamma-glutamyltranspeptidase (GGT) mRNA by screening a rat kidney library constructed in lambda gtll with antibodies specifically reactive to the enzyme protein. The clone selected an mRNA that was translated into a 62 Kd peptide, corresponding to the GGT precursor. The longest clone isolated was 1842 bp long with an open reading frame coding for...

The 70 kD heat shock cognate proteins (HSC70s) are constitutive members of the 70 kD heat shock related protein family. We have sequenced the cDNA of a bovine HSC70: clones were isolated from a bovine brain cDNA library in XgtlO (1) by hybridization with probes derived from a rat HSC70 cDNA clone (2); one full-length clone was sequenced. The deduced amino acid sequence matches CNBr-generated pe...

A repetitive DNA element was obtained from a crayfish genomic library while attempting to isolate the gene for the pigment concentrating hormone (PCH) with an oligonucleotide probe. The probe was directed to the coding region of the octapeptidic hormone deduced from the amino acid sequence of PCH (1). The isolated clone contained a 14 nucleotide sequence tandemly repeated 30 times; eleven nucle...

SOURCE/DESCRIPTION: A pancreatic polypeptide cDNA clone (465 base pair Insert 1n the vector pBR322) was isolated from a library created from human pancreatic neoplasm RNA. This cDNA clone spans a 95 amino acid sequence in the coding region with a 29 amino add amino terminus leader sequence, a three amino acid connecting sequence and a 27 amino add carboxyl terminus (Takeuchi and Yamada, 1985). ...