In this study, a rapid, precise, accurate, specific and sensitive ion-paired reverse phase liquid chromatographic method has been developed for the simultaneous estimation of nebivolol hydrochloride and valsartan in their capsule formulation. The chromatographic method was standardized using a HIQ sil C(18) column (250x4.6 mm i.d., 5 mum particle size) with UV detection at 289 nm and flow rate ...

A simple, sensitive, and inexpensive high-performance liquid-chromatographic method has been developed for simultaneous determination of hydrochlorothiazide and candesartan cilexetil in pharmaceutical formulations. Chromatographic separation was achieved on a Phenyl-2 column with a 25:75:0.2 mixture of 0.02 M potassium dihydrogen phosphate, methanol, and triethylamine, final pH 6.0 ± 0.1, as mo...

A novel open-tubular CEC column coated with chitosan-graft-(β-CD) (CDCS) was prepared using sol-gel technique. In the sol-gel approach, owing to the 3D network of sol-gel and the strong chemical bond between the stationary phase and the surface of capillary columns, good chromatographic characteristics and unique selectivity in separating isomers were shown. The column efficiencies of 55,000∼16...

In this study, 21 structurally diverse drugs with examined central nervous system penetration were used for prediction of blood-brain barrier penetration using HPLC-UV instrument. Drugs' chromatographic capacity factors were measured by the immobilized artificial membrane presented by phosphatidylcholine column. The correlation between chromatographic capacity factor, octanol-water partition co...

The use of supercritical fluids as chromatographic mobile phases provides rapid separations with high efficiency, favoring their use in enantioselective separations. Supercritical fluid technology (SFC) is a versatile tool in the purification, enantioseparation and large-scale production of enantiomers of pharmaceuticals over comparable liquid chromatographic methods and is gaining popularity i...

A Simple fast and precise reversed phase high performance liquid chromatographic method is developed for the Deflazacort drug. Chromatographic separation was performed a C18 column (250×4.6 mm) as a stationary phase with the mobile phase of Acetonitrile, methanol and water (90:5:5 v/v). Flow rate is 1.0 ml/min the detection of wave length is 230 nm. This method was validated for linearity, accu...

In this work we present a high performance micro gas chromatograph column with a novel two dimensional axial heating technique for faster and more precise temperature programming, resulting in an improved separation performance. Three different axial resistive heater designs were simulated theoretically on a 3.0 m × 300 μm × 50 μm column for the highest temperature gradient on a 22 by 22 μm col...

A high-performance liquid chromatographic method has been developed for the quantitative determination of piperacillin in plasma. The compound is first extracted from acid-treated plasma into chloroform: 1-pentanol (3:1) and reextracted into a small volume of an aqueous phase at pH 7, which is injected in the chromatographic column (microgram-Bondapack C18; 10 micrometers). The mobile phase is ...

Analytical methods published in the last 20 years for the extraction, chromatographic separation, and quantification of alkylphenol ethoxylates (APEO) and related compounds in environmental samples are reviewed. Examples of various isolation and preconcentration techniques for water, effluent, sediment and sludge are presented. This includes procedures from the classical liquid-liquid and Soxhl...

An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c is described. We use a 45-min incubation in acetate buffer (pH 5.5) to eliminate labile glycated hemoglobins. In this automated system conventional modules are used but it incorporates a solvent-switching valve to select either of two buffers, which differ in pH and NaCl concentration. The chromatographic column contai...