The development of drugs and diagnostics with desirable characteristics requires smart small-molecule ligandsligands with predefined binding parameters of interaction with the target. Here, we propose a general approach for selection of such ligands from highly diverse combinatorial libraries of small molecules by methods of kinetic capillary electrophoresis (KCE). We deduct three fundamental r...

The electrophoretic mobilities of three bacterial strains were investigated by capillary electrophoresis (CE) and were compared with results obtained by microelectrophoresis (ME). The CE measurements yielded bimodal electropherograms for two of the strains, thus illustrating for the first time that surface charge variations within a monoclonal population can be probed by CE. Intrapopulation var...

This paper reports the use of affinity capillary electrophoresis (ACE) in the measurement of binding constants involving low molecular weight receptors, using vancomycin as an example.2'3 In this system, it was straightforward to measure binding constants of four compounds (two pairs of enantiomeric peptides) to vancomycin simultaneously, and the technique can, in principle, be extended to larg...