Temperature-sensitive variants of Nicotiana tabacum were isolated from a liquid suspension culture of somatic cells by a negative selection procedure, using bromodeoxyuridine and light. A total of nine such variants have been recovered, with an estimated rate of 2 x 10(-7) per cell division. The appearance of the variants at the permissive temperature varied from nearly wild type, white and fri...

Growth-promoting action of simazine and other s-triazine herbicides was detected by the use of sorghum (Sorghum bicolor [L]. Moench) callus tissue and the chlorophyll retention test. Soil application of simazine [2-chloro-4, 6-bis(ethylamino)-s-triazine] at sublethal levels nearly doubled the growth-promoting action of sorghum root exudates. Treated plants yielded up to 26% more total protein t...

findings in relation to the inhibitory effects of natural substances against microorganisms showed that herbal products may be alternatives to synthetic and chemical drugs due to their significant therapeutic effects. the pennyroyal ( mentha pulegium ) as an important medicinal plant, belongs to the lamiaceae family is applied in the pharmaceutical and food industries. in recent years, plant ti...

To clarify the involvement of the arginine decarboxylase (ADC) pathway in the salt stress response, the polyamine titre, putrescine biosynthetic gene expression, and enzyme activities were investigated in apple [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.] in vitro callus under salt stress, during recovery after stress, and when ADC was inhibited by D-arginine, an inhibitor of AD...

Studies pertaining to the detection, isolation anid chemical characterization of naturally occurring and synthetic cytokinins have been aided by the utilization of cultured callus tissues derived from soybean cotyledons (6, 9, 10, 11, 12) and tobacco stem pith (7, 8, 13, 14). Both tissues, when exposed to a specific anid defined chemical milieu, require kinetin or another similarly active 6-sub...

INTRODUCTION Fracture repair constitutes the sequence of cell biological events following bone injury and recapitulates the steps of endochondral ossification observed during embryonic skeletal development and growth. Because of the different phases of fracture healing (inflammation, cartilage formation and remodeling) the fracture callus provides an excellent tool for analysis of cartilage and...

1. By the implantation of wedges containing indol-3-ylacetic acid and sucrose into blocks of undifferentiated bean-callus tissue it has been possible to induce the formation of xylem and phloem cells. 2. The differentiation has been investigated cytologically and measured chemically. 3. The optimum concentrations of the nutrients in the wedge, which gave differentiation closely resembling the v...

       In this study scion of Thompson seedless grape cultivar was grafted on three local cultivars (Chav-ga, Rash-meri and Tre-rash) rootstock by wedge grafting in 2021-2022, were made manually the 15th Febrywary using one-year old cuttings ( rootstock). Growth parameters such as shoot length diameter, dry weight vegetative growth, number leaves showed significant...