Borrelia burgdorferi the Lyme disease spirochete was grown in modified Kelly medium and characterized by transmission and by scanning electron microscopy. Using silver staining procedures which preferentially bind to nuclear components of eukaryotic cells, signal could be detected by backscattered electron imaging throughout the length of the prokaryotic spirochete. Interestingly, however, the ...

OBJECTIVE To evaluate the outcomes of conjunctival compression sutures (CCS) for enlarged overfiltering blebs associated with ocular hypotony after uncomplicated trabeculectomy with mitomycin C (TM). METHODS A retrospective analysis of consecutively recruited patients with enlarged conjunctival bleb associated with hypotony who had undergone CCS was performed. Patients were considered for CCS...

The ability to control and manipulate neuronal activity within an intact mammalian brain is of key importance for mapping functional connectivity and for dissecting the neural circuitry underlying behaviors. We have previously generated transgenic mice that express channelrhodopsin-2 for light-induced activation of neurons and mapping of neural circuits. Here we describe transgenic mice that ex...

PURPOSE The aim of this study was to analyze dry eye disease (DED) in patients with functioning filtering blebs and to explore the relationship between the morphology of filtering blebs and ocular surface instability. METHODS This was a cross-sectional, case-comparison study. Seventy glaucomatous patients (70 eyes) with functioning blebs who had undergone trabeculectomy more than 6 months pri...

Subcapsular sinus macrophages (SSMs) in lymph nodes are rapidly exposed to antigens arriving in afferent lymph and have a role in their capture and display to B cells. In tissue sections SSMs exhibit long cellular processes and express high amounts of CD169. Here, we show that many of the cells present in lymph node cell suspensions that stain for CD169 are not macrophages but lymphocytes that ...

RATIONALE Approximately 10% of patients who have a spontaneous pneumothorax have a positive family history. OBJECTIVES We sought to identify DNA sequence variations that confer susceptibility to pneumothoraces. METHODS We collected 12 families that had at least 2 first-degree relatives with a spontaneous pneumothorax. All affected family members had no obvious stigmata of known genetic diso...

ber bleb nevus syndrome (BRBNS) was admitted for occult bleeding. He had a history of gastric and colonic hemangiomas treated 2 years earlier by argon plasma coagulation and alcohol injection respectively. Clinical examination showed a few hemangiomas on his back and feet (●" Fig. 1). Capsule endoscopy (Pillcam SB2; Given Imaging, Yoqneam, Israel) disclosed three blebs of the small bowel (●" Fi...